Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

L-Selectin antibody (Biotin)

SELL Reactivity: Mouse FACS Host: Rat Monoclonal MEL-14 Biotin
Catalog No. ABIN114232
  • Target See all L-Selectin (SELL) Antibodies
    L-Selectin (SELL) (Selectin L (SELL))
    Reactivity
    • 99
    • 74
    • 31
    • 12
    • 9
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Mouse
    Host
    • 79
    • 55
    • 49
    Rat
    Clonality
    • 130
    • 48
    • 3
    Monoclonal
    Conjugate
    • 66
    • 26
    • 21
    • 14
    • 13
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This L-Selectin antibody is conjugated to Biotin
    Application
    • 135
    • 49
    • 48
    • 47
    • 35
    • 28
    • 16
    • 11
    • 11
    • 9
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    This monoclonal antibody reacts with a 90 kDa protein which is involved with the homing of lymphocytes to peripheral lymph nodes.
    Purification
    Protein G Chromatography
    Immunogen
    Mouse B cell Lymphoma, 38C-14 Donor: Fischer Rat Spleen Fusion Partner: P3 X 63Ag8.653
    Clone
    MEL-14
    Isotype
    IgG2a
    Top Product
    Discover our top product SELL Primary Antibody
  • Application Notes
    Flow cytometry. Immunohistochemistry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 1-0. 2 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 2 µg/10e6 cellsIsotypic Control: Biotin Rat IgG2aCell Source: Percentage of cells stained above controlThymus: 59. 8%Spleen: 66. 3%Lymph Node: 75. 8%
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    L-Selectin (SELL) (Selectin L (SELL))
    Alternative Name
    CD62L / L-Selectin (SELL Products)
    Synonyms
    CD62L antibody, LAM1 antibody, LECAM1 antibody, LEU8 antibody, LNHR antibody, LSEL antibody, LYAM1 antibody, PLNHR antibody, TQ1 antibody, A.11 antibody, L-selectin antibody, LECAM-1 antibody, SELE antibody, AI528707 antibody, Lnhr antibody, Ly-22 antibody, Ly-m22 antibody, Lyam-1 antibody, Lyam1 antibody, selectin L antibody, selectin, lymphocyte antibody, SELL antibody, Sell antibody
    Target Type
    Chemical
    Background
    L-selectin is expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils.1 Pre-incubation of lymphocytes with this antibody completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro2,3,6 and the migration of lymphocytes to lymph nodes in vivo.2,3 Polymorphonuclear cells preincubated with this antibody do not migrate to the inflammatory foci.Synonyms: CD62 antigen-like family member L, LAM-1, LECAM1, LNHR, LYAM1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL
    Gene ID
    20343
    NCBI Accession
    NP_035476
    UniProt
    P18337
You are here:
Support