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L-Selectin antibody (Selectin L)

Details for Product anti-SELL Antibody No. ABIN114235, Supplier: Log in to see
Antigen
  • A.11
  • AI528707
  • CD62L
  • L-selectin
  • LAM1
  • LECAM-1
  • LECAM1
  • LEU8
  • Lnhr
  • LNHR
  • LSEL
  • Ly-22
  • Ly-m22
  • Lyam-1
  • Lyam1
  • LYAM1
  • PLNHR
  • SELE
  • TQ1
Reactivity
Chemical
362
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74
23
10
1
1
1
Host
Rat
264
185
82
15
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This L-Selectin antibody is un-conjugated
92
77
34
32
13
8
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7
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4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
437
142
123
122
70
47
45
21
14
14
13
9
6
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5
3
2
2
2
2
2
2
2
2
1
1
1
Supplier
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Immunogen Mouse B cell Lymphoma, 38C-14. Donor: Fischer Rat Spleen Fusion Partner: P3 X 63Ag8.653
Clone MEL-14
Isotype IgG2a
Specificity This monoclonal antibody reacts with a 90 kDa protein which is involved with the homing of lymphocytes to peripheral lymph nodes.
Purification Protein G Chromatography
Alternative Name CD62L / L-Selectin (SELL Antibody Abstract)
Target Type Chemical
Background L-selectin is expressed on most T and B lymphocytes, neutrophils, monocytes, eosinophils.1 Pre-incubation of lymphocytes with this antibody completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro 2,3,6 and the migration of lymphocytes to lymph nodes in vivo.2,3 Polymorphonuclear cells preincubated with this antibody do not migrate to the inflammatory foci.Synonyms: CD62 antigen-like family member L, LAM-1, LECAM1, LNHR, LYAM1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL
Gene ID 20343
NCBI Accession NP_035476
UniProt P18337
Application Notes Flow cytometry. Immunohistochemistry. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 1-0. 2 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at 1: 700 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 2 µg/10e6 cellsIsotypic Control: Rat IgG2aCell Source: Percentage of cells stained above controlThymus: 85. 8%Spleen: 41. 0%Lymph Node: 75. 0%
Restrictions For Research Use only
Concentration 1,0 mg/mL
Buffer PBS and 0.02 % NaN3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-L-Selectin antibody (Selectin L) (ABIN114235) Rat anti CD62L (L-Selectin, LECAM-1) MEL-14
Background publications Siegelman, Cheng, Weissman, Wakeland: "The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding." in: Cell, Vol. 61, Issue 4, pp. 611-22, 1990

Lewinsohn, Bargatze, Butcher: "Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4313-21, 1987

Jalkanen, Bargatze, de los Toyos, Butcher: "Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells." in: The Journal of cell biology, Vol. 105, Issue 2, pp. 983-90, 1987

Fink, Gallatin, Reichert, Butcher, Weissman: "Homing receptor-bearing thymocytes, an immunocompetent cortical subpopulation." in: Nature, Vol. 313, Issue 5999, pp. 233-5, 1985

Reichert, Gallatin, Butcher, Weissman: "A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes." in: Cell, Vol. 38, Issue 1, pp. 89-99, 1984

Gallatin, Weissman, Butcher: "A cell-surface molecule involved in organ-specific homing of lymphocytes." in: Nature, Vol. 304, Issue 5921, pp. 30-4, 1983