You are viewing an incomplete version of our website. Please click to reload the website as full version.

CD90 antibody (THY1) (PE)

Details for Product anti-THY1 Antibody No. ABIN114256, Supplier: Log in to see
Antigen
  • CD90
  • CD7
  • T25
  • Thy-1
  • Thy-1.2
  • Thy1.1
  • Thy1.2
  • Thy-1 cell surface antigen
  • thymus cell antigen 1, theta
  • THY1
  • Thy1
Alternatives
anti-Mouse (Murine) CD90 antibody for Flow Cytometry
Reactivity
Mouse (Murine)
354
325
135
32
31
30
28
28
26
25
15
6
4
4
2
1
1
Host
Mouse
404
194
98
17
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD90 antibody is conjugated to PE
75
69
55
30
16
14
13
11
11
9
8
7
7
7
7
7
6
5
5
5
5
4
4
4
4
4
3
3
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
513
213
164
122
108
83
72
72
32
31
16
14
9
9
8
8
6
5
3
2
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen CBA/J. Donor: AKR/J Spleen. Fusion Partner: Spleen from immunized recipient fused with myeloma P3-NSI-1-Ag4-1.
Clone 5a-8
Isotype IgG2b
Specificity This monoclonal antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
Purification Protein G Chromatography
Alternative Name CD90 (THY1 Antibody Abstract)
Background CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
Gene ID 21838
NCBI Accession NP_033408
UniProt P01831
Research Area CD Antigens, Surface Receptors of Immune Cells, Experimental Models
Pathways Cell-Cell Junction Organization
Application Notes Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsIsotypic Control: PE-Mouse IgG2b,kCell Source Percentage of cells stained above control: Thymus: 98. 4%Spleen: 28. 8%Results - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATLNegative: AKR/J
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Supplier Images
Flow Cytometry (FACS) image for anti-CD90 antibody (THY1)  (PE) (ABIN114256) Cell Source: Thymus - Percentage of cells stained above control: 98.4%
Background publications Krieg, Gourley, Steinberg: "Association of murine lupus and thymic full-length endogenous retroviral expression maps to a bone marrow stem cell." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 9, pp. 3002-5, 1991 (PubMed).

Miyajima, Hirano, Hirose, Karasuyama, Okumura, Ovary: "Suppression by IL-2 of IgE production by B cells stimulated by IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 2, pp. 457-62, 1991 (PubMed).

Siegelman, Cheng, Weissman, Wakeland: "The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding." in: Cell, Vol. 61, Issue 4, pp. 611-22, 1990 (PubMed).

Kruger, Riley: "The age-dependent loss of bone marrow B cell precursors in autoimmune NZ mice results from decreased mitotic activity, but not from inherent stromal cell defects." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 1, pp. 103-10, 1990 (PubMed).

Fine, Silverstone, Gasiewicz: "Impairment of prothymocyte activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 4, pp. 1169-76, 1990 (PubMed).

Lewinsohn, Bargatze, Butcher: "Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4313-21, 1987 (PubMed).

Jalkanen, Bargatze, de los Toyos, Butcher: "Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells." in: The Journal of cell biology, Vol. 105, Issue 2, pp. 983-90, 1987 (PubMed).

Fink, Gallatin, Reichert, Butcher, Weissman: "Homing receptor-bearing thymocytes, an immunocompetent cortical subpopulation." in: Nature, Vol. 313, Issue 5999, pp. 233-5, 1985 (PubMed).

Reichert, Gallatin, Butcher, Weissman: "A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes." in: Cell, Vol. 38, Issue 1, pp. 89-99, 1984 (PubMed).

Gallatin, Weissman, Butcher: "A cell-surface molecule involved in organ-specific homing of lymphocytes." in: Nature, Vol. 304, Issue 5921, pp. 30-4, 1983 (PubMed).