Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody

Details for Product No. ABIN114275
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Antigen
Synonyms A830040C14Rik, AC-3, Apopain, CC3, CPP32, Caspase-3, Lice, Yama, mldy, Ly-6A.2, Ly-6A/E, Ly-6E.1, Sca-1, Sca1, TAP
Reactivity
Mouse (Murine)
(141), (20)
Host
Mouse
(122), (21), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(18), (16), (13), (12), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Cytotoxicity Test (CyTox)
(123), (32), (31), (10), (10), (9), (6), (5), (4), (2)
Pubmed 3 references available
Quantity 0.5 mL
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Catalog No. ABIN114275
544.50 $
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Clone 1147447
Isotype IgG2a
Specificity The Ly-6 antigens are a complex collection of determinants, probably coded for by a family of closely linked genes, referred to as Ly6A-E. These antigens have been shown to be differentially expressed on thymocytes, peripheral T and B cells, bone marrow cells, macrophages and neutrophils as well as nonhematopoietic tissues. (4) At present, there is little insight into the function of the products of the Ly-6 locus. (1) However, the antigens have been implicated in playing a role in T cell activation. (1) This mouse Ly6A.2 monoclonal antibody detects the classical Ly-6 alloantigen originally defined with conventional antiserum. (2) Ly6A.2 has been shown to be present on 50-70% of peripheral lymphocytes (including CD4+, CD8+ and B cells), on few thymocytes, all B and T cell blasts and is absent from most bone marrow cells and neutrophils. (2) Ly6A.2 has been located in close proximity to Ly6D.2 on the cell surface, and although related to one another, these two determinants are distinct from Ly6C.2 and Ly6B.2. (2) Thus, although the role of Ly6A.2 is unclear, antibodies to this antigen may be useful tools for separating functionally distinct subpopulations of lymphocytes and resolving pathways of differentiation. In fact, Ly6A.2 is absent from any precursor T cells, but is present on effector T cells. Anti-Ly6A.2 antibodies may also prove useful in T cell activation studies as one of the prominent features of the Ly6A.2 specificity is its anhanced expression following T Cell Activation. (1)
Purification Ascites
Alternative Name Ly6A/E / SCA1
Background Alternate names: Ly-6A.2/Ly-6E.1, Lymphocyte antigen 6A-2/6E-1, SCA-1, Stem cell antigen 1, T-cell-activating protein, TAP
Gene ID 110454
NCBI Accession NP_034868
UniProt P05533
Application Notes This antibody is a useful antibody for studies of myeloid differentiation stages and theirregulations by cytokines. Applications include flow cytometry (1,2,3) complement mediateddepletion (4) Western blot staining (5) and both frozen and paraffin sections (6). Otherapplications not tested. Optimal dilutions of this antibody are dependent on conditionsand should be determined by the user. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Two stage cytotoxicity as described above “Recommended method for determinationpercent of Ly6A. 2 positive lymphocytes”. C. I. = Cytotoxic Index=100 x % cyt (antibody + complement) - % cyt (complement alone)100% - % cyt (complement alone)
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70°C. Following reconstitution store the antibody (in aliquots) at -20°C for 6 month.
Expiry Date 12 months
Background publications Houlden, Hogarth, McKenzie: "Interrelationships of the \" antigens." in: Immunogenetics, Vol. 23, Issue 4, pp. 226-32, 1986 (PubMed).

Shevach, Korty: "Ly-6: a multigene family in search of a function." in: Immunology today, Vol. 10, Issue 6, pp. 195-200, 1989 (PubMed).

Sutton, Mickelson, Tobias et al.: "Identification of two forms of the Ly-6.2 antigen showing differential expression." in: Immunogenetics, Vol. 21, Issue 6, pp. 539-47, 1985 (PubMed).

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