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Lymphocyte Antigen 6 Complex, Locus A (Ly6a) antibody

Details for Product No. ABIN114275, Supplier: Login to see
Antigen
  • A830040C14Rik
  • AC-3
  • Apopain
  • CC3
  • CPP32
  • Caspase-3
  • Lice
  • Yama
  • mldy
  • Ly-6A.2
  • Ly-6A/E
  • Ly-6E.1
  • Sca-1
  • Sca1
  • TAP
Reactivity
Mouse (Murine)
179
12
3
Host
Mouse
168
14
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
26
21
18
14
9
7
5
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Cytotoxicity Test (CyTox)
164
47
46
38
14
8
6
6
5
4
3
2
2
2
2
1
1
1
Supplier
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Clone 1147447
Isotype IgG2a
Purification Ascites
Alternative Name Ly6A/E / SCA1 (Ly6a Antibody Abstract)
Background Synonyms: Ly-6A.2/Ly-6E.1, Lymphocyte antigen 6A-2/6E-1, SCA-1, Stem cell antigen 1, T-cell-activating protein, TAP
Gene ID 110454
NCBI Accession NP_034868
UniProt P05533
Pathways
Application Notes This antibody is a useful antibody for studies of myeloid differentiation stages and theirregulations by cytokines. Applications include flow cytometry (1,2,3) complement mediateddepletion (4) Western blot staining (5) and both frozen and paraffin sections (6).
Otherapplications not tested. Optimal dilutions of this antibody are dependent on conditionsand should be determined by the user.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Two stage cytotoxicity as described above “Recommended method for determinationpercent of Ly6A. 2 positive lymphocytes”. C. I. = Cytotoxic Index=100 x % cyt (antibody + complement) - % cyt (complement alone)100% - % cyt (complement alone)
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
Expiry Date 6 months
Background publications Sutton, Mickelson, Tobias et al.: "Identification of two forms of the Ly-6.2 antigen showing differential expression." in: Immunogenetics, Vol. 21, Issue 6, pp. 539-47, 1985 (PubMed).

Shevach, Korty: "Ly-6: a multigene family in search of a function." in: Immunology today, Vol. 10, Issue 6, pp. 195-200, 1989 (PubMed).

Houlden, Hogarth, McKenzie: "Interrelationships of the \' antigens." in: Immunogenetics, Vol. 23, Issue 4, pp. 226-32, 1986 (PubMed).