MHC Class I H-2Dd antibody (FITC)

Details for Product No. ABIN114288, Supplier: Log in to see
Antigen
Reactivity
Mouse (Murine)
21
Host
Mouse
21
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
4
3
2
Application
Flow Cytometry (FACS)
17
10
9
3
1
Options
Supplier
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Immunogen Recipient: C3H/HeJ Immunocyte Donor: B6 x DBA/2 spleen cells Fusion Partner: SP2/0.Ag14
Clone 34-5-8S
Isotype IgG2a
Specificity This anti-mouse H-2Dd monoclonal antibody is specific for cells expressing the H-2D antigen coded for by the d haplotype. The reaction pattern of this antibody with a panel of inbred and recombinant haplotypes demonstrates that the antibody detects a private determinant (H-2.4) of the H-2Dd antigen. This antibody can be used to quantitate cells bearing the H-2Dd(H-2.4) antigen from the appropriate strains of mice.
Purification Protein G Chromatography
Alternative Name MHC Class I H-2 Dd
Background Synonyms: D-D alpha chain, H-2 class I histocompatibility antigen, H-2D(D), H2-D1
Gene ID 100045864
NCBI Accession XP_003086970
UniProt P01900
Application Notes Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS:
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-MHC Class I H-2Dd antibody (FITC) (ABIN114288) FIGURE1: LFL1 - Cell Source: Spleen - Percentage of Cells Stained Above Control: 97.9%
Background publications Ozato, Mayer, Sachs: "Monoclonal antibodies to mouse major histocompatibility complex antigens." in: Transplantation, Vol. 34, Issue 3, pp. 113-20, 1982 (PubMed).

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