T Cell Receptor (TCR) Vb 4 antibody (FITC)

Antigen: T Cell Receptor (TCR) Vb 4

Clonality: Monoclonal (CTVB4)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN114336

Additional Information

Characteristics: Synonyms: TCR V beta-4, TCR Vb4

Isotype: IgG2a

Clone: CTVB4

Specificity: This antigen monoclonal antibody reats with TCR Vb4 bearing T cells. TCR Vb4 is expressedin most known mouse strains. Species: Mouse. Others not tested.

Application Details

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are

Application Notes: Flow Cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, 0.09% NaN3 and EIA grade BSA as a stabilizing protein to bring totalprotein concentration to 4-5 mg/ml.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should protected from light. Shelf life: one year from despatch.

Research Area: Immunology, Receptors, Signaling

Restrictions: For Research Use only

Images

Rat anti T-Cell Receptor TCR Vb 4 CTVB4