T Cell Receptor (TCR) Vb 4 antibody

Antigen: T Cell Receptor (TCR) Vb 4

Clonality: Monoclonal (CTVB4)

Host: Rat

Reactivity: Mouse (Murine)

Application: Flow Cytometry (FACS)

Catalog no.: ABIN114337

Additional Information

Characteristics: Synonyms: TCR V beta-4, TCR Vb4

Isotype: IgG2a  (Matching secondary antibodies)

Clone: CTVB4

Specificity: This monoclonal antibody reacts with TCR Vb4 bearing T cells. TCR Vb4 is expressed inmost known mouse strains. Species: Mouse. Others not tested.

Application Details

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C.

Application Notes: Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml

Buffer: PBS and contains 0.09% sodium azide (NaN3) as a preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Immunology, Receptors, Signaling

Restrictions: For Research Use only

Publications

Publications: Padula, Lingenheld, Stabach et al.: "Identification of encephalitogenic V beta-4-bearing T cells in SJL mice. Further evidence for the V region disease hypothesis?" in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 3, pp. 879-83, 1991 (PubMed).

Tomonari, Lovering, Spencer: "Correlation between the V beta 4+ CD8+ T-cell population and the H-2d haplotype." in: Immunogenetics, Vol. 31, Issue 5-6, pp. 333-9, 1990 (PubMed).