T Cell Receptor (TCR) Vb 8.3 antibody (FITC)

Antigen: T Cell Receptor (TCR) Vb 8.3

Clonality: Monoclonal (KT-8C1)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN114339

Additional Information

Characteristics: Synonyms: TCR V beta-8.3, TCR Vb8.3

Isotype: IgG2a

Clone: KT-8C1

Specificity: This monoclonal antibody detects TCR Vb 8. 3 bearing T cells. Species: Mouse. Others not tested.

Application Details

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most flurochromes are light sensitive. )7. Wash 2 times at 4°C.

Application Notes: Flow Cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, 0.09% NaN3 and EIA grade BSA as a stabilizing protein to bring totalprotein concentration to 4-5 mg/ml.Label: FITC

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This antibody is photosensitive and should protected from light. Shelf life: one year from despatch.

Research Area: Immunology, Receptors

Restrictions: For Research Use only

Images

Mouse anti T-Cell Receptor TCR Vb 8.3 KT-8C1