T Cell Receptor (TCR) Vb 10b antibody (PE)

Antigen: T Cell Receptor (TCR) Vb 10b

Clonality: Monoclonal (CTVB10b)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN114348

Additional Information

Characteristics: Synonyms: TCR V beta-10b, TCR Vb10b

Isotype: IgG2b

Clone: CTVB10b

Specificity: This antibody reacts with the TCR Vβ10b bearing T cells of mice expressing the b haplotype(ie. A, AKR, Balb/c) of the TCRb gene complex. This antibody will not react with mice havingthe a (ie. C57BR, C57L, SJL, SWR) or c (ie. RIII) TCRb haplotype. Species: Mouse. Others not tested.

Application Details

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg of this antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells.

Application Notes: Flow Cytometry (protocol see below). Appropriate control samples should always be included in any labelling studies. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, 0.09 % NaN3 and EIA grade BSA as a stabilizing protein to bring totalprotein concentration to 4-5 mg/mlLabel: PE

Storage: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

Research Area: Immunology, Receptors, Signaling

Restrictions: For Research Use only

Images

Rat anti T-Cell Receptor TCR Vb 10b CTVB10b