T Cell Receptor (TCR) Vb 12 antibody (FITC)

Antigen: T Cell Receptor (TCR) Vb 12

Clonality: Monoclonal (CTVB12b)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN114354

Additional Information

Characteristics: Synonyms: TCR V beta-12, TCR Vb12

Isotype: IgG2a

Clone: CTVB12b

Specificity: This monoclonal antibody reacts with TCR Vb12b bearing cells. Species: Mouse. Others not tested.

Application Details

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B.

Application Notes: Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf life: one year from despatch

Research Area: Immunology, Receptors, Signaling

Restrictions: For Research Use only

Images

Rat anti T-Cell Receptor TCR Vb 12 CTVB12b