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Antibodies
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anti-T Cell Receptor (TCR) Vb 12 antibody
T Cell Receptor (TCR) Vb 12 antibody
| Antigen |
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| Clonality |
Monoclonal (CTVB12b) |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN114355 |
| Quantity |
0.2 mg (0.2 mg/ml) |
| Price |
340.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: TCR V beta-12, TCR Vb12
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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CTVB12b
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Specificity
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This monoclonal antibody reacts with TCR Vb12b bearing cells. Species: Mouse. Others not tested.
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Protocol
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FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium (CL5030). 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x106 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of CL7212AP. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody CLCC40001 (FITC Goat anti-rat IgG (H+L)) at 1: 500dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are
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Application Notes
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Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.2 mg/ml
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Buffer
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PBS and 0.09% NaN3
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch
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Research Area
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Immunology, Receptors, Signaling
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Restrictions
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For Research Use only
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Alternatives for antigen "T Cell Receptor (TCR) Vb 12", type "Antibodies"
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