Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CD44 antibody (Biotin)

CD44 Reactivity: Rat FACS, WB, IHC (p), IHC (fro) Host: Mouse Monoclonal OX-49 Biotin
Catalog No. ABIN114362
  • Target See all CD44 Antibodies
    CD44
    Reactivity
    • 292
    • 152
    • 81
    • 30
    • 25
    • 24
    • 16
    • 11
    • 9
    • 9
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 168
    • 123
    • 77
    • 2
    • 1
    Mouse
    Clonality
    • 253
    • 116
    • 2
    Monoclonal
    Conjugate
    • 164
    • 42
    • 30
    • 26
    • 18
    • 8
    • 8
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD44 antibody is conjugated to Biotin
    Application
    • 260
    • 153
    • 91
    • 73
    • 70
    • 66
    • 60
    • 40
    • 34
    • 31
    • 29
    • 13
    • 7
    • 7
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This monoclonal antibody recognizes rat CD44 (Pgp-1), also called CD44H. This antigen is expressed on most leukocytes (except a sub population of B cells) and increases upon activation. The OX-49 antibody binds extracellularly to the standard (S) form on rat leukocytes but it is not known if they bind to the N-terminal region. It has also been reported that the antibody may bind to melanoma cell lines that express CD44V (splice variant form).
    Purification
    Protein G Chromatography
    Immunogen
    T cell blasts. Donor: BALB/c spleen. Fusion Partner: myeloma cell line NSO/1.
    Clone
    OX-49
    Isotype
    IgG2a
    Top Product
    Discover our top product CD44 Primary Antibody
  • Application Notes
    Flow Cytometry. Western Blotting. Immunohistochemistry on frozen and paraffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 1. 0-0. 5 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at 1: 500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: WistarCell Concentration: 1 x 10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsIsotypic Control: Biotin Mouse IgG2aCell Source Percentage of cells stained above control:
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    CD44
    Alternative Name
    CD44 (CD44 Products)
    Synonyms
    CDW44 antibody, CSPG8 antibody, ECMR-III antibody, HCELL antibody, HUTCH-I antibody, IN antibody, LHR antibody, MC56 antibody, MDU2 antibody, MDU3 antibody, MIC4 antibody, Pgp1 antibody, AU023126 antibody, AW121933 antibody, AW146109 antibody, HERMES antibody, Ly-24 antibody, Pgp-1 antibody, CD44A antibody, METAA antibody, RHAMM antibody, XCD44 antibody, cd44 antibody, cdw44 antibody, cspg8 antibody, ecmr-iii antibody, hcell antibody, lhr antibody, mc56 antibody, mdu2 antibody, mdu3 antibody, mic4 antibody, mutch-i antibody, pgp1 antibody, CD44 molecule (Indian blood group) antibody, syndecan 4 antibody, CD44 antigen antibody, CD44 molecule (Indian blood group) S homeolog antibody, CD44 antibody, SDC4 antibody, Cd44 antibody, cd44.S antibody
    Background
    CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1
    Gene ID
    10116
    UniProt
    P26051
    Pathways
    Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
You are here:
Support