Asialoganglioside GM1 antibody

Antigen: Asialoganglioside GM1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN114402

Additional Information

Characteristics: Synonyms: Asialo GM1, GA1, Ganglioside asialo GM1

Immunogen: Asialo GM1 purified from bovine brain tissue, methylated bovine serum albumin andcomplete Freund's adjuvant.

Description: Gangliosides are neuraminic acid containing glycosphingolipids and representcharacteristic constituents of the plasma membrane of eukaryotic cells. They are shed inthe microenvironment and found as free components in plasma. In turn, free gangliosidesare efficiently incorporated into the plasma cell membrane.

Specificity: Anti-Asialo GM1 polyclonal antibody reacts with Natural Killer (NK) cells. It also exhibits slight reactivity with mouse monocytes (liver cells which contain no NKcells, bone marrow, fetal liver cells, spleen cells of nude mice), macrophages, and fetalthymocytes (12 days old, ratio of existence decreased gradually until there were none innewborn mice). Anti-Asialo GM1 antiserum has been shown to eliminate NK activity in cells of variousstrains of mice and rats. Species: Mouse and Rat. Others not tested. Add. Information: Ig classes of the antibody: IgG, IgA, and IgM.

Application Details

Protocol: Procedure For Measurement of Anti-NK Cell ActivityIN VITRO: 1. Preparation of target cells: - Suspend 5 x 10e6 cells of YAC-1 in RPMI 1640 containing 10% FCS. 2. Preparation of effector cells: - Inject 0. 2 ml of polyinosinic- polycytidylic acid soduim salt solution (500 µg per ml of polyI: C in RPMI 1640) into BALB/c mice. - Remove spleens from mice on the following day (after approximately 10 hours oftreatment) and prepare spleen cell suspension as follows: - Centrifuge at 1000 rpm for 10 minutes and discard supernatant. - Add 0. 83% NH4Cl to the tube to hemolyze the precipitated spleen cells. - Centrifuge at 1000 rpm for 10 minutes and discard supernatant. Add 10 ml RPMI 1640 towash cells. Repeat washing process using same procedure. - Adjust the cell number to 2. 5 x 10e7 cells per ml.

Application Notes: Flow Cytometry. Immunohistochemistry (Frozen and Paraffin Embedded Sections). Immunoprecipitation. NK Cell Depletion. Injections: Mouse - intravenously: 10-50 µl (approximately 20 µl, 50 injections can be made using 20µl doses). The exact dosage should be decided from titration data (see Protocols) andthe nature of the study. The first injection may be effective for 4 days with a gradualdiminution. Therefore, 3-4 injections are necessary for a 2 week study. (Incubation)Days / Injection: 0 / 1st5 / 2nd10 / 3rd14 / 4thRat - intravenously:

Concentration: Antibody Titre: approximately 1/1000 by immunoflocculation test.Total Protein 38 mg/ml.Albumin 8 mg/ml.

Storage: Store the antibody at 2-8°C before reconstitution. After reconstitution store at 2-8° for up to 2 months. DO NOT FREEZE. Recontstituted product is stable for 2 days at RT. Shelf life: one year from despatch.

Research Area: Metabolism

Restrictions: For Research Use only

Images

Titration of Anti-Asialo GM1 in vitro

Publications

Publications: Kasai, Iwamori, Nagai et al.: "A glycolipid on the surface of mouse natural killer cells." in: European journal of immunology, Vol. 10, Issue 3, pp. 175-80, 1980 (PubMed).

Habu, Fukui, Shimamura et al.: "In vivo effects of anti-asialo GM1. I. Reduction of NK activity and enhancement of transplanted tumor growth in nude mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 1, pp. 34-8, 1981 (PubMed).