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Mouse anti-Human Ig (Light Chain), (Chain kappa) Antibody

Details for Product No. ABIN114482, Supplier: Log in to see
Antigen
Epitope
Light Chain, Chain kappa
577
447
323
77
26
13
12
7
6
5
5
4
2
2
1
Reactivity
Human
696
227
77
34
19
13
10
5
5
5
5
5
4
2
2
1
1
1
1
1
1
1
1
1
Host
Mouse
430
385
165
84
21
9
4
1
1
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Immunogen Purified Bence Jones kappa light chain protein
Clone KP-53
Isotype IgG2b
Purification Supernatant
Alternative Name Immunoglobulin
Background Immunoglobulins belong to a group of related glyco proteins which make up 20 % of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit, while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
Research Area Secondary Antibodies, Virology
Application Notes Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/50-1/100 in anABC method (30 minutes at room temperature). Although not required, enzymatic predigestion may help in some cases. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Hernandez, Frierson, Moskaluk, Li, Clegg, Cote, McCusker, Hankey, Edwards, Goodman: "CK20 and CK7 protein expression in colorectal cancer: demonstration of the utility of a population-based tissue microarray." in: Human pathology, Vol. 36, Issue 3, pp. 275-81, 2005

Baars, De Ruijter, Smedts, Van Niekerk, Poels, Seldenrijk, Ramaekers: "The applicability of a keratin 7 monoclonal antibody in routinely Papanicolaou-stained cytologic specimens for the differential diagnosis of carcinomas." in: American journal of clinical pathology, Vol. 101, Issue 3, pp. 257-61, 1994