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kappa Light Chain antibody

Details for Product No. ABIN114482
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Antigen
Synonyms LOC100037688
Reactivity
Human
(121), (27), (14), (2)
Host
Mouse
(61), (53), (25), (21), (4), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(20), (10), (10), (9), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(64), (55), (51), (46), (32), (32), (27), (13), (10), (10), (9), (5), (2), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 1 mL
Shipping to United States (Change)
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Catalog No. ABIN114482
308.00 $
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Immunogen Purified Bence Jones kappa light chain protein
Clone KP-53
Isotype IgG2b
Specificity This antibody reacts with B cell follicles in human lymphoid tissues. The mantle zones give a mosaic pattern, while the germinal centers show a coarse meshwork pattern of staining. The antibody stains free as well as bound kappa light chains. It does not react with human lambda light chain. Cellular localization: Cytoplasm.
Purification Supernatant
Background Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit, while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
Research Area Secondary Antibodies
Application Notes Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/50-1/100 in anABC method (30 minutes at room temperature). Although not required, enzymatic predigestion may help in some cases. Recommended Positive Control: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
General Baars, De Ruijter, Smedts et al.: "The applicability of a keratin 7 monoclonal antibody in routinely Papanicolaou-stained cytologic specimens for the differential diagnosis of carcinomas." in: American journal of clinical pathology, Vol. 101, Issue 3, pp. 257-61, 1994 (PubMed).

Hernandez, Frierson, Moskaluk et al.: "CK20 and CK7 protein expression in colorectal cancer: demonstration of the utility of a population-based tissue microarray." in: Human pathology, Vol. 36, Issue 3, pp. 275-81, 2005 (PubMed).

Validation Images
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