Melanoma antibody

Antigen: Melanoma

Clonality: Monoclonal (HMB 45)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Catalog no.: ABIN114489

Additional Information

Immunogen: BALB/C mice were injected with extract of pigmented melanoma metastases from lymphnodes.

Cross-Reactivity: Human

Isotype: IgG1

Clone: HMB 45

Specificity: This antibody reacts with a neuraminidase sensitive oligosaccharide side chain of aglycoconjugate present in immature melanosomes. It reacts with junctional and blue nevuscells. Non-melanocytic cells are negative. Cellular Localization: Cytoplasm. Species: Human. Others not tested.

Application Details

Application Notes: Western Blot. Formalin fixed Paraffin Embedded Tissue: 1/25-1/50 in an ABC method for 30 minutes atroom temperature. Positive Control: Melanoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Supernatant

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer

Restrictions: For Research Use only

Images

Formalin fixed paraffin embedded human melanoma stained with Melanoma antibody ABIN114488/ABIN114488-05

Publications

Kapur, Bigler, Skelly et al.: "Anti-melanoma monoclonal antibody HMB45 identifies an oncofetal glycoconjugate associated with immature melanosomes." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 40, Issue 2, pp. 207-12, 1992 (PubMed).

Gown, Vogel, Hoak et al.: "Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes." in: The American journal of pathology, Vol. 123, Issue 2, pp. 195-203, 1986 (PubMed).

Lee, Guo, Lim et al.: "Clinical and molecular characteristics of hereditary non-polyposis colorectal cancer families in Southeast Asia." in: Clinical genetics, Vol. 68, Issue 2, pp. 137-45, 2005 (PubMed).