CD68 Molecule (CD68) antibody

Details for Product No. ABIN114505
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Antigen
Synonyms Lamp4, Scard1, gp110, GP110, LAMP4, SCARD1
Reactivity
Human
(119), (68), (47), (20), (3), (2), (1), (1)
Host
Mouse
(90), (76), (39)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(29), (14), (12), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(103), (75), (69), (45), (25), (20), (20), (19), (17), (13), (10), (4), (1), (1)
Pubmed 2 references available
Quantity 1 mL
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Catalog No. ABIN114505
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Immunogen BALB/C mice were injected with Gaucher cells.
Clone PG-M1
Isotype IgG3
Specificity CD68 antibody Clone PG-M1 reacts strongly with a fixative-resistant epitope of an intracytoplasmic molecule, selectively expressed by virtually all macrophages of the human body. Although attempts to immunoprecipitate the molecule recognized by PG-M1 have failed so far, the reactivity of the antibody with COS-1 and WOP cells transfected with a human complementary DNA clone encoding for the CD68 antigen suggests that PG-M1 is a new member of the CD68 cluster. However, unlike other CD68 antibodies (KP1, EBM11, etc.), which react with both macrophages and myeloid cells, PG-M1 detects a fixative- resistant epitope on the macrophage-restricted form of the CD68 antigen. In 957 routinely fixed, paraffin-embedded samples, PG-M1 showed a more restricted reactivity with elements of the monocyte/macrophage lineage than the previously described monoclonal antibodies MAC-387 (anti- calgranulins), KP1 (CD68) and Ki-M1P. Among hematological malignancies, PG-M1 only labels acute leukemias of M4 and M5 type and rare examples of malignant histiocytosis/true histiocytic sarcoma. In contrast, acute leukemias of the M1, M2, M3, M6, M7, and L1-L3 types, non-Hodgkin's lymphomas, and Hodgkin and Reed- Sternberg cells of Hodgkin's disease are consistently PG-M1-negative. In the daily diagnostic practice, PG- M1 seems to be particularly valuable for the diagnosis of myelomonocytic or monocytic leukemia and neoplasms of true histiocytic origin in routine paraffin sections (see Ref. 4).
Purification Supernatant
Alternative Name CD68
Background The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ- specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
Alternate names: Gp110, Macrophage marker, Macrosialin
Gene ID 968
NCBI Accession NP_001035148
UniProt P34810
Research Area Immunology, CD Antigens, Surface Receptors of Immune Cells
Application Notes Immunohistochemistry on paraffin embedded sections: dilute 1: 10-1: 40 in an ABC method(30 minutes at room temperature). Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embeddedtissue sections. PG-M1 is not recommended for frozen sections or cell smears. Positive control: TonsilOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer Sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
General Clos, Westwood, Becker et al.: "Molecular cloning and expression of a hexameric Drosophila heat shock factor subject to negative regulation." in: Cell, Vol. 63, Issue 5, pp. 1085-97, 1991 (PubMed).

Baler, Dahl, Voellmy: "Activation of human heat shock genes is accompanied by oligomerization, modification, and rapid translocation of heat shock transcription factor HSF1." in: Molecular and cellular biology, Vol. 13, Issue 4, pp. 2486-96, 1993 (PubMed).

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