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Prostate Specific Antigen antibody

PSA Reactivity: Human WB, IHC (p), IHC (fro), EIA, ELISA (Capture) Host: Mouse Monoclonal CHYH1 unconjugated
Catalog No. ABIN2853579
  • Target See all Prostate Specific Antigen (PSA) Antibodies
    Prostate Specific Antigen (PSA)
    Reactivity
    • 189
    • 15
    • 10
    • 5
    • 4
    • 1
    Human
    Host
    • 117
    • 65
    • 4
    • 4
    • 2
    Mouse
    Clonality
    • 131
    • 61
    Monoclonal
    Conjugate
    • 101
    • 17
    • 13
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Prostate Specific Antigen antibody is un-conjugated
    Application
    • 90
    • 77
    • 37
    • 36
    • 26
    • 25
    • 25
    • 13
    • 13
    • 9
    • 9
    • 8
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), ELISA (Capture)
    Specificity
    DM1035 reacts with PSA. This antibody is reactive to normal prostate, benign prostate hyperplasia and prostate malignant carcinoma. It has not reacted with other normal tissues tested.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human.
    Purification
    Affinity Chromatography on Protein G
    Immunogen
    Human Prostate Specific Antigen
    Clone
    CHYH1
    Isotype
    IgG1
    Top Product
    Discover our top product PSA Primary Antibody
  • Application Notes
    ELISA: In sandwich ELISA this antibody can be used as a Capture antibody in combinationwith a Monoclonal tracer/detection antibody (HRP conjugate, TMB as substrate). Recommended Capture Coating Dose: 0.5-1.0 μg/well. Under the suggested conditionsapproximately 1 ng/mL of PSA in human serum can be detected with an assay range of0-200 ng/mL. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections (ABC method). Western blot: 10 μg/mL allows visualization of 1 μg/lane human PSA.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with double distilled water to a final concentration of 1.0 mg/mL.
    Buffer
    0.01 M PBS, pH 7.0 without preservatives
    Preservative
    Without preservative
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • Target
    Prostate Specific Antigen (PSA)
    Alternative Name
    Kallikrein-3 / PSA / KLK3 (PSA Products)
    Synonyms
    APS antibody, KLK2A1 antibody, PSA antibody, hK3 antibody, KLK3 antibody, Klk1c10l2 antibody, RSGK-50 antibody, RSKG-50 antibody, rGK-3 antibody, Kal-3 antibody, Kal3 antibody, Klk3 antibody, AAP-S antibody, MP100 antibody, Psa antibody, R74825 antibody, goku antibody, kallikrein related peptidase 3 antibody, kallikrein 3 antibody, kallikrein 1-related peptidase C3 antibody, kallikrein B, plasma 1 antibody, aminopeptidase puromycin sensitive antibody, KLK3 antibody, Klk1c3 antibody, Klkb1 antibody, Npepps antibody
    Background
    Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors.Synonyms: Gamma-seminoprotein, P-30 antigen, Prostate-specific antigen, Semenogelase
    Gene ID
    354
    NCBI Accession
    NP_001025218
    UniProt
    P07288
    Pathways
    Complement System
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