pan Cytokeratin antibody

Antigen: pan Cytokeratin

Clonality: Monoclonal (Cocktail)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN114573

Additional Information

Alternative name:

Immunogen: Cytokeratins from human breast carcinoma cell line MCF-7 and detergent insoluble fractionof psoriatic epidermis.

Cross-Reactivity: Human

Isotype: Cocktail

Clone: Cocktail

Description: This antibody is a cocktail of two different clones, 5D3 andLP53.

Characteristics: Synonyms: pan Keratin, Cytokeratin Pan-reactive

Application Details

Application Notes: Immunohistochemistry on paraffin embedded sections: diluted of 1: 15-1: 50 in an ABCmethod (30 minutes at room temperature)Proteolytic digestion of tissue sections is required prior to immunostaining. Positive Control: SkinOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Supernatant

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Angus, Kiberu, Purvis et al.: "Cytokeratins in cervical dysplasia and neoplasia: a comparative study of immunohistochemical staining using monoclonal antibodies NCL-5D3, CAM 5.2, and PKK1." in: The Journal of pathology, Vol. 155, Issue 1, pp. 71-5, 1988 (PubMed).

Gatter, Abdulaziz, Beverley et al.: "Use of monoclonal antibodies for the histopathological diagnosis of human malignancy." in: Journal of clinical pathology, Vol. 35, Issue 11, pp. 1253-67, 1983 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).