Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) antibody

Antigen: Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5)

Synonyms: CEA, CD66e, DKFZp781M2392, Psg30, 1600029H12Rik, CEACAM5, CEACAM

Clonality: Monoclonal (26-3-13)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS)

Catalog no.: ABIN114595

Additional Information

Alternative name: CD66e / CEACAM5

UniProt: P06731

Immunogen: Extracted protein of CEACAM5

Cross-Reactivity: Human

Isotype: IgG1

Clone: 26-3-13

Description: CEACAM5 (CEA-related cell adhesion molecule 5, CEA) belongs to the carcinoembryonicantigen (CEA) gene family (1,2). It encodes a glycosyl phosphatidyl inositol (GPI)-linkedglycoprotein with a Mr of 180,000-200,000 which is most strongly expressed on epithelialcells of the fetal and adult colon and to a minor extent on epithelial cells of the stomachand sweat glands, squamous epithelial cell of the tongue, esophagus and cervix. CEACAM5is used as a tumor marker for early detection of recurrent disease due to its expression inadenocarcinomas of the colon, lung, breast, stomach and pancreas and in mucinousovarian carcinomas (3). Like all members of the CEACAM family, it consists of a single Ndomain, with structural homology to the immunoglobulin variable domains, followed by siximmunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3).

Characteristics: Synonyms: CEA, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule5, Meconium antigen 100

Application Details

Application Notes: Flow Cytometry: 1.2 µg/10e6 cells. ELISA: 1/200-1/400. Immunoflourescence: 1/200. Western blot: 4 µg/ml. Immunohistochemistry on Frozen Sections: 1-2 µg/10e6 cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 2.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.2 without preservatives.

Storage: Store the antibody at 2-8°C for up to one week or (in aliquots) at -20°C to -80°C for longer. We recommend a shock-freeze procedure using liquid nitrogen for freezing. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer, Receptors, Immunology, Cell/Tissue Markers, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Strobel, Strobel, Bross et al.: "Effects of human bone marrow stroma on the growth of human tumor cells." in: Cancer research, Vol. 49, Issue 4, pp. 1001-7, 1989 (PubMed).

Grunert, AbuHarfeil, Schwarz et al.: "Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns." in: International journal of cancer. Journal international du cancer, Vol. 36, Issue 3, pp. 357-62, 1985 (PubMed).

Thompson, Moessinger, Reichardt et al.: "A polymerase-chain-reaction assay for the specific identification of transcripts encoded by individual carcinoembryonic antigen (CEA)-gene-family members." in: International journal of cancer. Journal international du cancer, Vol. 55, Issue 2, pp. 311-9, 1993 (PubMed).

Krop-Watorek, Laskowska, Salwa et al.: "CEA-related proteins on human urothelial cell lines of different transformation grades." in: Cancer letters, Vol. 139, Issue 1, pp. 15-22, 1999 (PubMed).

Schoelzel, Zimmermann, Schwarzkopf et al.: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, 2000 (PubMed).

Baczy?ska, Wietrzyk, Madej et al.: "The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen (CEACAM5) expression." in: Cellular & molecular biology letters, Vol. 8, Issue 2, pp. 471-86, 2003 (PubMed).