B-Cell CLL/lymphoma 2 (BCL2) antibody

Details for Product No. ABIN114625
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(606), (173), (129), (13), (7), (3), (3), (3), (3), (1), (1)
(473), (182), (20), (7), (2)
Clonality (Clone)
Monoclonal ()
(32), (27), (24), (23), (18), (15), (7), (7), (7), (7), (7), (7), (7), (7), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(482), (265), (226), (144), (143), (113), (99), (70), (40), (40), (35), (22), (21), (13), (10), (10), (5), (4), (2), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 0.5 mL
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Catalog No. ABIN114625
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Immunogen BALB/C mice were injected with synthetic bcl-2 peptide.
Clone 100-D5
Isotype IgG1
Specificity The bcl-2 product is considered to act as an inhibitor of apoptosis. bcl-2 is expression is inhibited in germinal centers where apoptosis forms part of B cell production pathway. Reactive follicle show no staining for bcl-2, where as the cells in neoplastic follicles exhibit membrane staining. Cellular Localization: Cell membrane
Purification Purified
Alternative Name Bcl-2
Background BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.
Alternate names: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Gene ID 596
NCBI Accession NP_000624
UniProt P10415
Research Area Cancer, Apoptosis/Necrosis
Application Notes Immunoprecipitation: 1/50Western blot: 1/50-1/100Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABCmethod (30-60 minutes at room temperature)Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with Citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-B-Cell CLL/lymphoma 2 (BCL2) antibody Western blot analysis of human lung cell extract with Bcl2 antibody. ABIN114624
Background publications Hockenbery, Nuñez, Milliman et al.: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).

General Liu, Mason, Johnson et al.: "Germinal center cells express bcl-2 protein after activation by signals which prevent their entry into apoptosis." in: European journal of immunology, Vol. 21, Issue 8, pp. 1905-10, 1991 (PubMed).

LeBrun, Kamel, Cleary et al.: "Follicular lymphomas of the gastrointestinal tract. Pathologic features in 31 cases and bcl-2 oncogenic protein expression." in: The American journal of pathology, Vol. 140, Issue 6, pp. 1327-35, 1992 (PubMed).

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