Cyclin E1 (CCNE1) antibody
| Antigen |
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| Synonyms |
CCNE, AW538188, Ccne, CYCLE, cycE1, cyce2, cycE3, MGC85048, CCNE1, ccne1 |
| Clonality |
Monoclonal (13A3) |
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Host
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Reactivity
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Conjugate
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Biotin (4), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Application
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 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (79), Immunofluorescence (IF) (57), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (42), ELISA (41), Immunoprecipitation (IP) (35), Immunohistochemistry (IHC) (25), Flow Cytometry (FACS) (13), Immunocytochemistry (ICC) (9), Functional Studies (Func) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Gel Shift (GS) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Kinase Activity Assay (KAA) (1)
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| Catalog no. |
ABIN114668 |
| Quantity |
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| Price |
363.00 $ Plus shipping costs $45.00
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| Shipping to |
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| Availability |
Will be delivered in 6 to 8 Business Days |
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Alternative name
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Cyclin E1
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UniProt
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P24864
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Immunogen
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Recombinant full length cyclin E molecule. Genename: CCNE1
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Cross-Reactivity
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Human
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Isotype
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IgG2a
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Clone
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13A3
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Description
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Cyclin proteins are a family of related proteins which control the progression of theeukaryotic cell cycle. Overexpression of cyclin e shortens the length of the G1 phase,accelerating progression of S phase. The activity of cyclin E is mediated through itsactivation of cyclin dependent kinase 2 (cdk2) protein and is modulated by the presence oftumor suppressor proteins such as p16.
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Characteristics
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Synonyms: Cyclin-E1, CCNE1, CCNE
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Application Notes
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Immunoprecipitation (Native and Denatured): Use Protein A and Ab at 20 µl/mg proteinlysate)Immunohistology (Formalin/Paraffin): 1/20-1/40 for 0 min at RT. Staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Positive Control: Breast carcinoma. Western blot: A doublet of 50kDa and a single band of 42kDa is observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Purification
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Supernatant
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Cell Cycle
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Restrictions
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For Research Use only
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Alternatives for antigen "Cyclin E1 (CCNE1)", type "Antibodies"
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Hosts
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Rabbit (102), Mouse (37), Sheep (1)
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Reactivities
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Human (112), Mouse (Murine) (45), Rat (Rattus) (41), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Monkey (1)
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Applications
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Western Blotting (WB) (79), Immunofluorescence (IF) (57), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (42), ELISA (41), Immunoprecipitation (IP) (35), Immunohistochemistry (IHC) (25), Flow Cytometry (FACS) (13), Immunocytochemistry (ICC) (9), Functional Studies (Func) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Gel Shift (GS) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Kinase Activity Assay (KAA) (1)
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Conjugates
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Biotin (4), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Epitopes
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pThr395 (11), C-Term (9), AA 43-52 (5), pThr62 (5), Internal Region (3), Center (1), pThr286 (1), pThr75 (1), pThr77 (1)
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2013 BioMed Central Research Awards
