Cyclin E1 (CCNE1) antibody

Details for Product No. ABIN114668
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Antigen
Synonyms CCNE1, ccne, cyclinE, CCNE, AW538188, CycE1, CYCLE, Ccne, cyce2, cycE3, cyce, fa18e07, wu:fa18e07
Reactivity
Human
(239), (41), (36), (8), (1)
Host
Mouse
(216), (44), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(161), (76), (73), (72), (66), (40), (37), (31), (19), (14), (6), (6), (5), (3), (2), (1), (1), (1)
Pubmed 7 references available
Quantity 0.5 mL
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Catalog No. ABIN114668
390.50 $
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Immunogen Recombinant full length cyclin E molecule. Genename: CCNE1
Clone 13A3
Isotype IgG2a
Specificity This antibody reacts with a protein of 50 kd known as Cyclin E or Cyclin E1. Cellular localization: Nuclear.
Purification Supernatant
Alternative Name Cyclin E1
Background Cyclin proteins are a family of related proteins which control the progression of the eukaryotic cell cycle. Overexpression of cyclin e shortens the length of the G1 phase, accelerating progression of S phase. The activity of cyclin E is mediated through its activation of cyclin dependent kinase 2 (cdk2) protein and is modulated by the presence of tumor suppressor proteins such as p16.Synonyms: CCNE, CCNE1, Cyclin-E1
Gene ID 898
NCBI Accession NP_001229
UniProt P24864
Research Area Cell Cycle
Application Notes Immunoprecipitation (Native and Denatured): Use Protein A and Ab at 20 μL/mg proteinlysate)Immunohistology (Formalin/Paraffin): 1/20-1/40 for 0 min at RT. Staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Positive Control: Breast carcinoma. Western blot: A doublet of 50 kDa and a single band of 40 kDa is observed.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
anti-Cyclin E1 (CCNE1) antibody Formalin-fixed, paraffin-embedded human placenta stained with Cyclin E Ab (Cat. ABIN114667) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of trophoblastic cells.
Background publications Keyomarsi, OLeary, Molnar et al.: "Cyclin E, a potential prognostic marker for breast cancer." in: Cancer research, Vol. 54, Issue 2, pp. 380-5, 1994 (PubMed).

Gong, Traganos, Darzynkiewicz: "Simultaneous analysis of cell cycle kinetics at two different DNA ploidy levels based on DNA content and cyclin B measurements." in: Cancer research, Vol. 53, Issue 21, pp. 5096-9, 1993 (PubMed).

Faha, Harlow, Lees: "The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2." in: Journal of virology, Vol. 67, Issue 5, pp. 2456-65, 1993 (PubMed).

General Dulić, Lees, Reed: "Association of human cyclin E with a periodic G1-S phase protein kinase." in: Science (New York, N.Y.), Vol. 257, Issue 5078, pp. 1958-61, 1992 (PubMed).

Koff, Cross, Fisher et al.: "Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family." in: Cell, Vol. 66, Issue 6, pp. 1217-28, 1991 (PubMed).

Ohtsubo, Theodoras, Schumacher et al.: "Human cyclin E, a nuclear protein essential for the G1-to-S phase transition." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2612-24, 1995 (PubMed).

Sewing, Roenicke, Buerger et al.: "Alternative splicing of human cyclin E." in: Journal of cell science, Vol. 107 ( Pt 2), pp. 581-8, 1994 (PubMed).

Validation Images
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