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EPCAM antibody (Epithelial Cell Adhesion Molecule)

Details for Product anti-EPCAM Antibody No. ABIN114701, Supplier: Log in to see
Antigen
  • CD326
  • cd326
  • co17-1a
  • DIAR5
  • EGP
  • egp
  • EGP-2
  • egp40
  • EGP40
  • Egp314
  • EGP314
  • ep-cam
  • Ep-CAM
  • EPCAM
  • EpCAM1
  • ESA
  • GA733-2
  • ga733-2
  • gp40
  • GP40
  • hegp-2
  • HNPCC8
  • KS1/4
  • KSA
  • ksa
  • LEU-9
  • Ly74
  • M4S1
  • m4s1
  • MGC80540
  • mic18
  • MIC18
  • MK-1
  • mk-1
  • sb:cb6
  • Tacsd1
  • tacstd
  • Tacstd1
  • tacstd1
  • TACSTD1
  • Tp40
  • TP41
  • TROP1
  • trop1
  • wu:fj17g02
  • zgc:77119
  • zgc:110304
Reactivity
Human
742
118
68
2
2
1
1
1
Host
Mouse
631
133
41
1
Clonality (Clone)
Monoclonal ()
Conjugate
This EPCAM antibody is un-conjugated
41
37
28
17
17
17
14
13
13
13
12
12
12
12
12
12
12
12
12
6
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
558
454
365
225
200
171
108
99
83
12
8
5
5
4
4
3
2
2
2
1
1
1
1
1
Supplier
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Supplier Product No.
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Immunogen Recombinant human EPCAM produced in E.coli
Clone MK-1-25
Isotype IgG1
Specificity The anti human EPCAM antibody, clone MK-1-25 is a mouse monoclonal antibody against recombinant human EPCAM.
Purification Purified
Alternative Name CD326 / EPCAM / TACSTD1 (EPCAM Antibody Abstract)
Background The Ep-CAM antigen (Epithelial Cell Adhesion Molecule), also termed as MK-1, GA733-2, KSA, 17-1A or EGP40, is a 40 kDa type-I transmembrane glycoprotein with properties of Ca2+ -independent homotypic intracellular adhesion molecule. The full leghth of the molecule is 314 amino acids. Structurally, Ep-CAM is not related to any of the major families of adhesion molecules. The Ep-CAM antigen is overexpessed on the majority of tumor cells of most human epithelia of variety of tumor tissues such as stomach, colon, pancreas, gall bladder, bile duct, mammary gland, breast and lung carcinoma. Ep-CAM has been suggested to be involved in the differentiation and growth of epithelial cells under normal physiological conditions through its homotypic cell-cell adhesion activity. As it is overexpressed on most carcinomas, Ep-CAM antigen has been used as a target for diagnosis and therapy of cancer.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
Gene ID 4072
NCBI Accession NP_002345
UniProt P16422
Research Area Cancer
Application Notes ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.1 mL of deionized water
Concentration 1.0 mg/mL
Buffer 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE
Preservative Azide free
Storage 4 °C/-20 °C
Storage Comment Store at 2-8 °C for up to one month. For longer storage, aliquot and freeze unused portion at -20 °C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Background publications Abe, Kuroki, Imakiire, Yamauchi, Yamada, Arakawa, Kuroki: "Preparation of recombinant MK-1/Ep-CAM and establishment of an ELISA system for determining soluble MK-1/Ep-CAM levels in sera of cancer patients." in: Journal of immunological methods, Vol. 270, Issue 2, pp. 227-33, 2002 (PubMed).

Balzar, Briaire-de Bruijn, Rees-Bakker, Prins, Helfrich, de Leij, Riethmüller, Alberti, Warnaar, Fleuren, Litvinov: "Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions." in: Molecular and cellular biology, Vol. 21, Issue 7, pp. 2570-80, 2001 (PubMed).

Balzar, Winter, de Boer, Litvinov: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).