CCL7 antibody

Catalog No. ABIN114719

Product Details anti-CCL7 Antibody

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Reactive with natural and recombinant Human MCP-3. Does not show any cross reaction with other Human Cytokines or growth factors tested such as IL1 beta, IL-8, MCAF, SAA and EGF.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: Recombinant human MCP-3 (M.W. 8.5 kDa containing 76 amino acids residues)

Clone: 542

Isotype: IgG2a

Application Details

Application Notes: ELISA: CCL7 antibody can be used as a Capture antibody in Sandwich ELISA applicationsfor Human MCP-3 Detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 3 μg/well. Substrate: TMB. Western Blot: An antibody concentration of 0.5-2 μg/mL will allow visualization of 0.1 μg/lane of Human MCP-3.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated to adjust the final concentration to 1.00 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. DO NOT FREEZE!

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: MCP3 / CCL7 (CCL7 Products)

Synonyms: CCL7 antibody, FIC antibody, MARC antibody, MCP-3 antibody, MCP3 antibody, NC28 antibody, SCYA6 antibody, SCYA7 antibody, Scya7 antibody, fic antibody, marc antibody, mcp3 antibody, C-C motif chemokine ligand 7 antibody, chemokine (C-C motif) ligand 7 antibody, CCL7 antibody, Ccl7 antibody

Background: Macrophage/monocyte chemotactic protein-3 (MCP-3), like other two closely related chemokines (MCP1 and MCP-2), is chemotactic to monocytes, but not to neutrophils. MCP-3 showed 78 % sequence homology with MCP-1 and 58 % sequence homology with MCP-2. The primary receptor for MCP-3 is D6. MCP-3 also competes with MCP-1 to bind to CCR10, and competes with Rantes and MIP-1α to bind to CCR1. MCP-3 stimulates macrophage to secrete proteases. A variety of cancer cell lines express MCP-3, suggesting a role in tumor invasion and metastasia.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7

Gene ID: 6354

NCBI Accession: NP_006264

UniProt: P80098