Collagen IV antibody (Collagen, Type IV)

Details for Product anti-COL4 Antibody No. ABIN114747, Supplier: Log in to see
Antigen
  • 1209
  • 6072
  • CG16858
  • CT25584
  • Coll IValpha2
  • DmColA2
  • Dmel\\CG16858
  • Vkg
  • VkgC
  • alpha(IV)2/vkg
  • col4a2
  • coll. IV
  • l(2)01209
  • CG25C
  • CG4145
  • CT12803
  • Cgc25
  • Cgc25C
  • Coll
  • Coll IV
  • DCg1
  • Dcg
  • Dcg1
  • Dmel\\CG4145
  • alpha(IV)1/Cg25C
  • alpha1
  • cg25C
  • cg25c
  • col4a1
  • gp125
  • viking
  • Collagen type IV
  • collagen, type IV
  • vkg
  • Cg25C
  • Let-2
Reactivity
Human
234
67
54
32
15
9
3
2
2
2
2
2
1
1
1
1
Host
Mouse
135
101
27
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Collagen IV antibody is un-conjugated
19
9
5
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
141
101
98
89
82
53
26
22
21
21
20
13
5
2
2
2
1
1
1
Options
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Immunogen Human collagen type IV.
Clone COL-94
Isotype IgG1
Specificity This antibody recognizes collagen type IV epitope located on the alpha1 and/or alpha 2 chains of human collagen type IV. This antibody shows no cross reactivity with collagen types I, II, III, V, VI and VII as well as does not react with human vitronectin, fibronectin or chondroitin sulfate A, B and C.
Cellular Localization: Basement membrane
Purification Supernatant
Alternative Name Collagen Type IV (COL4 Antibody Abstract)
Background Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.Synonyms: COL4A1
Gene ID 1282
NCBI Accession NP_001836
UniProt P02462
Research Area Extracellular Matrix
Application Notes Immunohistochemistry on formalin-fixed paraffin embedded sections: diluted of 1: 25-1: 50in an ABC method (30 minutes at room temperature)Recommended Positive Control: Skin
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Sanes, Engvall, Butkowski, Hunter: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).

Sage, Woodbury, Bornstein: "Structural studies on human type IV collagen." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9893-900, 1979 (PubMed).