Actin (pan) antibody

Antigen: Actin

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Epitope: pan

Clonality: Monoclonal (1A4-5C5)

Host: Mouse

Reactivity: Cow (Bovine), Guinea Pig, Human, Rabbit, Rat (Rattus), Sheep (Ovine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN114753

Additional Information

Alternative name: Actin (pan alpha)

Immunogen: BALB/C mice were injected with N-terminal decapeptide of alpha-smooth muscle actin for1A4 and with purified rabbit striated muscle actin for 5C5.

Format: Ascites

Clone: 1A4-5C5

Specificity: This pan actin is useful in identifying tumors arising from muscle cells, i. e. leiomyosarcoma as well as rhabdomyosarcoma. Cellular Localization: CytoplasmicSpecies: Human, Sheep, Bovine, Rabbit, Pig, Rat, Frog, Snake, Carp. Others not tested.

Synonyms: A, Ac5C, Act, Actin, Actin/BAP47, Actin5C, BAP47, M32055, T11, act 5C, act5C, actin-5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, DmelCG4027, CG4027, Act57A, Act57b, BEST:RE38067, C, CG30294, M, T10, act 57A, anon-EST:CL2c12, DmelCG10067, CG10067, 143060_f_at, D, act 79B, act79B, mus79B, DmelCG7478, CG7478, Act(88F), Act88-F, E, Ifm(3)7, act 88F, act88F, rsd, DmelCG5178, CG5178, act 87E, act87E, mus87E, DmelCG18290, CG18290, ACTIN, ACTIN-12, actin, dsim_GLEANR_2829, DsimGD19025, GD19025, Act88F, AFFX-Dros-ACTIN_M_r_at, Act42a, act 42A, DmelCG12051, CG12051

Application Details

Application Notes: Immunohistochemistry on formalin-fixed paraffin embedded sections: diluted of 1: 25-1: 50in an ABC method (30 minutes at room temperature)Recommended Positive Control: Skeletal muscleOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.125 mg/ml

Buffer: containing sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Publications

Publications: Schuerch, Skalli, Seemayer et al.: "Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. I. Smooth muscle tumors." in: The American journal of pathology, Vol. 128, Issue 1, pp. 91-103, 1987 (PubMed).

Babai, Skalli, Schurch et al.: "Chemically induced rhabdomyosarcomas in rats. Ultrastructural, immunohistochemical, biochemical features and expression of alpha-actin isoforms." in: Virchows Archiv. B, Cell pathology including molecular pathology, Vol. 55, Issue 5, pp. 263-77, 1988 (PubMed).

Skalli, Gabbiani, Babaui et al.: "Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. II. Rhabdomyosarcomas." in: The American journal of pathology, Vol. 130, Issue 3, pp. 515-31, 1988 (PubMed).