anti-Cyclin E1 antibody (CCNE1)

Details for Product anti-CCNE1 Antibody No. ABIN114787
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Synonyms CCNE1, ccne, cyclinE, CCNE, AW538188, CycE1, CYCLE, Ccne, cyce2, cycE3, cyce, fa18e07, wu:fa18e07
(308), (53), (44), (7), (2), (2), (2), (2), (1)
(262), (67), (2)
Clonality (Clone)
Monoclonal ()
This Cyclin E1 antibody is un-conjugated
(11), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(207), (107), (92), (85), (76), (50), (40), (30), (13), (10), (7), (7), (6), (4), (3), (2), (1), (1), (1)
Pubmed 7 references available
Quantity 0.5 mL
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide corresponding to human cyclin E protein.
Clone HE12
Isotype IgG1
Specificity This antibody recognizes a doublet of 50 kDa and a single band of 42 kDa identified as Cyclin E.
Cellular Localization: Nuclear
Purification Supernatant
Alternative Name Cyclin E1 (CCNE1 Antibody Abstract)
Background Cyclin proteins are a family of related proteins, which control the progression of the eukaryotic cell cycle. Overexpression of cyclin E shortens the length of the G1 phase, accelerating progression of S phase. The activity of cyclin E is mediated through its activation of cyclin dependent kinase 2 (cdk2) protein and is modulated by the presence of tumor suppressor proteins such as p16. During tumor cell and normal cell proliferation, cyclins activate the cyclin-dependent protein kinases, which in turn controls progression through the G1 phase of the cell cycle.Synonyms: CCNE, CCNE1, Cyclin-E1
Gene ID 898
NCBI Accession NP_001229
UniProt P24864
Research Area Cell Cycle
Pathways Cell Division Cycle
Application Notes Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in anABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (IHC) image for anti-Cyclin E1 antibody (CCNE1) (ABIN114787) Formalin fixed paraffin embedded human breast carcinoma stained with Cyclin E antibod...
Background publications Gong, Sun, Michael et al.: "Immunocytochemistry of serous effusion specimens: a comparison of ThinPrep vs cell block." in: Diagnostic cytopathology, Vol. 28, Issue 1, pp. 1-5, 2002 (PubMed).

Faha, Harlow, Lees: "The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2." in: Journal of virology, Vol. 67, Issue 5, pp. 2456-65, 1993 (PubMed).

Sewing, Roenicke, Buerger et al.: "Alternative splicing of human cyclin E." in: Journal of cell science, Vol. 107 ( Pt 2), pp. 581-8, 1994 (PubMed).

Baker, Sanger, Rodgers et al.: "Cell proliferation kinetics of normal and tumour tissue in vitro: quiescent reproductive cells and the cycling reproductive fraction." in: Cell proliferation, Vol. 28, Issue 1, pp. 1-15, 1995 (PubMed).

Ohtsubo, Theodoras, Schumacher et al.: "Human cyclin E, a nuclear protein essential for the G1-to-S phase transition." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2612-24, 1995 (PubMed).

Koff, Cross, Fisher et al.: "Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family." in: Cell, Vol. 66, Issue 6, pp. 1217-28, 1991 (PubMed).

Dulić, Lees, Reed: "Association of human cyclin E with a periodic G1-S phase protein kinase." in: Science (New York, N.Y.), Vol. 257, Issue 5078, pp. 1958-61, 1992 (PubMed).

Catalog No. ABIN114787
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