TAG72 antibody

Antigen: TAG72

Clonality: Monoclonal (CC49)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN114821

Additional Information

Characteristics: Synonyms: CA72-4, CA 72-4, sialy-Tn

Immunogen: BALB/C mice were injected with purified TAG-72 protein.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: CC49

Description: This antibody has been shown to be distinct from B72. 3 by reciprocal competitionradio-immunoassays and it has a Ka higher than that of B72. 3. TAG-72 is expressed incolon, breast, pancreatic, ovarian, lung and gastric cancer.

Specificity: This antibody is specific to an oncofetal antigen of >1,000 kD known as tumor associatedglycoprotein (TAG-72) or CA 72-4. This antibody has been shown to be distinct from B72. 3 by reciprocal competitionradio-immunoassays and it has a Ka higher than that of B72. 3. TAG-72 is expressed in colon, breast, pancreatic, ovarian, lung and gastric cancer. Cellular localization: Cytoplasm, cell surface. Species: Human. Others not tested.

Application Details

Application Notes: Immunohistochemistry on formalin-fixed paraffin embedded sections: dilute 1: 50-1: 100 inan ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended positive control: Breast carcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

Formalin fixed paraffin embedded human colon carcinoma stained with TAG-72 antibody ABIN114820

Publications

Publications: Katari, Fernsten, Schlom: "Characterization of the shed form of the human tumor-associated glycoprotein (TAG-72) from serous effusions of patients with different types of carcinomas." in: Cancer research, Vol. 50, Issue 16, pp. 4885-90, 1990 (PubMed).

Sheer, Schlom, Cooper: "Purification and composition of the human tumor-associated glycoprotein (TAG-72) defined by monoclonal antibodies CC49 and B72.3." in: Cancer research, Vol. 48, Issue 23, pp. 6811-8, 1988 (PubMed).

Colcher, Minelli, Roselli et al.: "Radioimmunolocalization of human carcinoma xenografts with B72.3 second generation monoclonal antibodies." in: Cancer research, Vol. 48, Issue 16, pp. 4597-603, 1988 (PubMed).