This Thymidine Phosphorylase antibody is un-conjugated
Application
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody is specific to a 55 kDa protein known as Platelet-Derived Endothelial Growth Factor (PD-ECGF), similar to thymidine phosphorylase (TP). Cellular localization: Cytoplasmic, Nuclear.
Purification
Purified
Immunogen
A recombinant full length human thymidine phophorylase (TP/PD-ECGF) protein.
Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of1/25-1/75 in an ABC method (30 minutes at room temperature). Western blotting: 1/50-1/100. Recommended Positive Control: Breast carcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Platelet - derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites. High levels of TP/PD-ECGF are observed in cancer patients. High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.Synonyms: ECGF1, Gliostatin, PD-ECGF, Platelet-derived endothelial cell growth factor, TdRPase