KRT15 antibody (C-Term)

Catalog No. ABIN114890

Product Details anti-KRT15 Antibody

Target: KRT15 (Keratin 15 (KRT15))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT15 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Cytokeratin 15 is a type I cytokeratin without a defined type II partner.
Cellular Localization: Cytoplasmic.

Purification: Purified

Immunogen: A 17-mer synthetic peptide from the C-terminus of human cytokeratin 15

Clone: LHK15

Isotype: IgG2a

Application Details

Application Notes: Immunoflourescence. Immunohistochemistry on Paraffin Embedded Sections: 1/25-1/75 dilution in an ABCmethod (30 minutes at room temperature). Requires high temperature antigen unmaskingwith 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KRT15

Target: KRT15 (Keratin 15 (KRT15))

Alternative Name: Cytokeratin 15 (KRT15 Products)

Synonyms: xlk2 antibody, CK15 antibody, K15 antibody, K1CO antibody, AI528832 antibody, Krt1-15 antibody, Ka15 antibody, wu:fa91c06 antibody, wu:fa95d04 antibody, zgc:55334 antibody, keratin 15, type I L homeolog antibody, keratin 15, type I antibody, keratin 15 antibody, krt15.L antibody, krt15 antibody, KRT15 antibody, Krt15 antibody

Background: Cytokeratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues. Cytokeratin 15 (CK15) is a type I keratin without a defined type II partner whose expression in epidermal diseases has not been investigated. A monoclonal antibody raised against the last 17 amino acids of the CK15 polypeptide show that CK-15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Although CK-15 in normal hair follicles was virtually absent from hair bulbs, it was expressed by a subset of keratinocytes in the outer root sheath. In human conjunctival epithelium, strong expression of CK-15 was observed in basal cells, whereas CK19 was expressed in both basal and suprabasal layers. Although the expression of K15 and K19 differ in humans and mice, specific staining patterns can be used to characterize the epithelial phenotype in normal and diseased ocular surface.Synonyms: CK-15, Cytokeratin-15, K15, KRT15, KRTB, Keratin, Keratin 15, Keratin-15, Type I Cytoskeletal 15

Gene ID: 3866

NCBI Accession: NP_002266

UniProt: P19012