Prolactin-Induced Protein (PIP) antibody

Antigen: Prolactin-Induced Protein (PIP)

Clonality: Monoclonal (23A3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN114914

Additional Information

Alternative name: GCDFP15

UniProt: P12273

Immunogen: Recombinant protein encoding the excreted domain of human GCDFP-15

Cross-Reactivity: Human, Rat (Rattus)

Isotype: IgG2a

Clone: 23A3

Description: Gross Cystic Disease Fluid Protein-15 (GCDFP-15) is a 15 kD monomer protein. Gross cysticdisease of the breast is benign premenopausal disorder in which cysts are predominant. These cysts appear to be formed from excessive apocrine cystic secretions, one of which isGCDFP-15.

Characteristics: Synonyms: PIP, GCDFP-15, GPIP4, SABP, gp17, SMGP, Secretory Actin-Binding Protein,Prolactin-Inducible Protein, Gross Cystic Disease Fluid Protein 15

Specificity: This antibody is specific to 15 kD monomer protein called Gross Cystic Disease FluidProtein-15 (GCDFP-15). Gross cystic disease of the breast is benign premenopausal disorderin which cysts are predominant. These cysts appear to be formed from excessive apocrinecystic secretions, one of which is GCDFP-15. This antibody can be used in for the staining ofbreast carcinoma, salivary duct carcinoma and apocrine epithelia. Cellular localization: Cytoplasmic. Species: Human. Others not tested.

Application Details

Application Notes: Immunohistochemistry: 1/10-1/20 in an ABC method. Incubation period: 60 minutes atroom temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Supernatant

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Formalin fixed paraffin embedded human skin stained with GCDFP15 antibody ABIN114912

Publications

Viacava, Naccarato, Bevilacqua: "Spectrum of GCDFP-15 expression in human fetal and adult normal tissues." in: Virchows Archiv : an international journal of pathology, Vol. 432, Issue 3, pp. 255-60, 1998 (PubMed).

Hall, Clements, Birrell et al.: "Prostate-specific antigen and gross cystic disease fluid protein-15 are co-expressed in androgen receptor-positive breast tumours." in: British journal of cancer, Vol. 78, Issue 3, pp. 360-5, 1998 (PubMed).