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Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Antigen

Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)
Synonyms HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a
Clonality Monoclonal (OZ12)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Electrophoretic Mobility-Shift Assay (EMSA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Catalog no. ABIN114960
Quantity
Price 352.00 $   Plus shipping costs $45.00
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anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Additional Information
Application Details Images Alternatives Variants back to top

Alternative name HIF1A / HIF1 alpha
UniProt Q16665
Immunogen hHIF-1 alpha (aa 530-826) protein
Cross-Reactivity Human
Isotype IgG1
Clone OZ12
Description HIF-1 is a key component of a widely operative transcriptional response activated byhypoxia, cobaltous ions and iron chelation. HIF-1 activates transcription ofhypoxia-inducible genes.
Characteristics Synonyms: HIF-1 alpha, Hypoxia-inducible factor 1 alpha, ARNT-interacting protein, Member of PASprotein 1, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helixprotein 78
Specificity This antibody is specific to a 120 kD protein HIF-1, which is a heterodimer consisting of analpha and a beta subunits. Cellular Localization: Nuclear. Species: Human. Others not tested.

Application Details
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Application Notes Gel Supershift. Western Blot: 1/100-1/500. Immunofluorescence. Recommended Positive Control: Mammalian cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.1 mg/ml
Purification Purified
Storage Store the antibody undiluted at 2-8°CShelf life: One year from despatch.
Restrictions For Research Use only

Alternatives
Application Details Images Variants back to top

Alternatives for antigen "Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)", type "Antibodies"
Hosts Rabbit (83), Mouse (50)
Reactivities Human (104), Mouse (Murine) (40), Rat (Rattus) (37), Cow (Bovine) (32), Chicken (23), Pig (Porcine) (21), Dog (Canine) (20), Monkey (6), Ferret (3), Sheep (Ovine) (3), Rabbit (2), Cat (Feline) (1), Hamster (1), Primates (1)
Applications Western Blotting (WB) (86), ELISA (39), Immunofluorescence (IF) (39), Immunohistochemistry (IHC) (34), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (30), Immunoprecipitation (IP) (15), Gel Shift (GS) (11), Immunocytochemistry (ICC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Chromatin Immunoprecipitation (ChiP) (2), Electrophoretic Mobility-Shift Assay (EMSA) (2), Immunohistochemistry (Fixed) (IHC (fx)) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (10), FITC (3), HRP (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes C-Term (9), AA 329-530 (7), AA 432-528 (7), AA 530-826 (7), N-Term (4), AA 400-550 (2), AA 530-825 (2), AA 609-826 (2), Internal Region (2), OH-Pro564 (2), AA 775-790 (1), Center (1), Pro564 (1)