Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 530-826) antibody

Details for Product No. ABIN114960
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Antigen
Synonyms HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hif1a, HIF1-alpha, HIF-1a, HIF1a, hif-1a
Epitope
AA 530-826
(40), (36), (22), (9), (8), (7), (7), (7), (6), (6), (6), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(258), (118), (79), (56), (38), (34), (20), (19), (17), (16), (13), (2), (2), (2), (1), (1)
Host
Mouse
(188), (127), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(28), (15), (12), (12), (12), (8), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Electrophoretic Mobility-Shift Assay (EMSA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(243), (98), (94), (83), (74), (67), (51), (37), (12), (11), (10), (9), (9), (3), (3), (1)
Pubmed 2 references available
Catalog no. ABIN114960
Quantity 0.5 mL
Price
379.50 $   Plus shipping costs $45.00
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen hHIF-1 alpha (aa 530-826) protein
Clone OZ12
Isotype IgG1
Specificity This antibody is specific to a 120 kD protein HIF-1, which is a heterodimer consisting of an alpha and a beta subunits. Cellular Localization: Nuclear.
Purification Purified
Alternative Name HIF1A / HIF1 alpha
Background HIF-1 is a key component of a widely operative transcriptional response activated by hypoxia, cobaltous ions and iron chelation. HIF-1 activates transcription of hypoxia-inducible genes.
Alternate names: ARNT-interacting protein, BHLHE78, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, HIF-1 alpha, Hypoxia-inducible factor 1 alpha, Member of PAS protein 1
Gene ID 3091
NCBI Accession NP_001521.1
UniProt Q16665
Application Notes Gel Supershift. Western Blot: 1/100-1/500. Immunofluorescence. Recommended Positive Control: Mammalian cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 530-826) antibody Western blot analysis of human lung cell
General Arany, Huang, Eckner et al.: "An essential role for p300/CBP in the cellular response to hypoxia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 23, pp. 12969-73, 1996 (PubMed).

Huang, Arany, Livingston et al.: "Activation of hypoxia-inducible transcription factor depends primarily upon redox-sensitive stabilization of its alpha subunit." in: The Journal of biological chemistry, Vol. 271, Issue 50, pp. 32253-9, 1997 (PubMed).

Hosts (188), (127), (3), (1)
Reactivities (258), (118), (79), (56), (38), (34), (20), (19), (17), (16), (13), (2), (2), (2), (1), (1)
Applications (243), (98), (94), (83), (74), (67), (51), (37), (12), (11), (10), (9), (9), (3), (3), (1)
Conjugates (28), (15), (12), (12), (12), (8), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (40), (36), (22), (9), (8), (7), (7), (7), (6), (6), (6), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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