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Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 530-826) antibody

Details for Product No. ABIN114960, Supplier: Log in to see
Antigen
  • hif1a
  • AA959795
  • HIF1alpha
  • MOP1
  • bHLHe78
  • HIF-1A
  • HIF-1alpha
  • HIF1
  • HIF1-ALPHA
  • PASD8
  • HIF1-alpha
  • hif-1a
Epitope
AA 530-826
44
39
17
15
15
13
13
9
8
8
6
6
6
5
5
5
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
299
118
88
39
21
9
8
7
5
3
3
1
1
1
1
1
1
1
1
1
1
Host
Mouse
228
142
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
27
19
9
7
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Electrophoretic Mobility-Shift Assay (EMSA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
247
162
106
88
74
56
31
25
23
11
10
7
6
3
3
3
2
2
2
2
Supplier
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Immunogen hHIF-1 alpha (aa 530-826) protein
Clone OZ12
Isotype IgG1
Specificity This antibody is specific to a 120 kDa protein HIF-1, which is a heterodimer consisting of an alpha and a beta subunits.
Cellular Localization: Nuclear.
Purification Purified
Alternative Name HIF1A / HIF1 alpha (HIF1A Antibody Abstract)
Background HIF-1 is a key component of a widely operative transcriptional response activated by hypoxia, cobaltous ions and iron chelation. HIF-1 activates transcription of hypoxia-inducible genes.Synonyms: ARNT-interacting protein, BHLHE78, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, HIF-1 alpha, Hypoxia-inducible factor 1 alpha, Member of PAS protein 1
Gene ID 3091
NCBI Accession NP_001521
UniProt Q16665
Pathways
Application Notes Gel Supershift. Western Blot: 1/100-1/500. Immunofluorescence. Recommended Positive Control: Mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 530-826) antibody (ABIN114960) Western blot analysis of human lung cell
Background publications Huang, Arany, Livingston et al.: "Activation of hypoxia-inducible transcription factor depends primarily upon redox-sensitive stabilization of its alpha subunit." in: The Journal of biological chemistry, Vol. 271, Issue 50, pp. 32253-9, 1997 (PubMed).

Arany, Huang, Eckner et al.: "An essential role for p300/CBP in the cellular response to hypoxia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 23, pp. 12969-73, 1996 (PubMed).