Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 530-826) antibody

Details for Product No. ABIN114960, Supplier: Log in to see
Antigen
  • hif1a
  • AA959795
  • HIF1alpha
  • MOP1
  • bHLHe78
  • HIF-1A
  • HIF-1alpha
  • HIF1
  • HIF1-ALPHA
  • PASD8
  • HIF1-alpha
  • hif-1a
  • hypoxia inducible factor 1 alpha
  • hypoxia inducible factor 1, alpha subunit
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • hypoxia-inducible factor 1 alpha
  • hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • Protein HIF-1
  • hif-1a
  • Hif1a
  • HIF1A
  • hif-1
Alternatives
anti-Human Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) antibody for Electrophoretic Mobility-Shift Assay
Epitope
AA 530-826
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1
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Reactivity
Human
611
304
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145
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28
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9
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Host
Mouse
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277
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
52
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25
18
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Application
Electrophoretic Mobility-Shift Assay (EMSA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen hHIF-1 alpha (aa 530-826) protein
Clone OZ12
Isotype IgG1
Specificity This antibody is specific to a 120 kDa protein HIF-1, which is a heterodimer consisting of an alpha and a beta subunits.
Cellular Localization: Nuclear.
Purification Purified
Alternative Name HIF1A / HIF1 alpha (HIF1A Antibody Abstract)
Background HIF-1 is a key component of a widely operative transcriptional response activated by hypoxia, cobaltous ions and iron chelation. HIF-1 activates transcription of hypoxia-inducible genes.Synonyms: ARNT-interacting protein, BHLHE78, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, HIF-1 alpha, Hypoxia-inducible factor 1 alpha, Member of PAS protein 1
Gene ID 3091
NCBI Accession NP_001521
UniProt Q16665
Pathways Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Application Notes Gel Supershift. Western Blot: 1/100-1/500. Immunofluorescence. Recommended Positive Control: Mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 530-826) antibody (ABIN114960) Western blot analysis of human lung cell
Background publications Huang, Arany, Livingston, Bunn: "Activation of hypoxia-inducible transcription factor depends primarily upon redox-sensitive stabilization of its alpha subunit." in: The Journal of biological chemistry, Vol. 271, Issue 50, pp. 32253-9, 1997 (PubMed).

Arany, Huang, Eckner, Bhattacharya, Jiang, Goldberg, Bunn, Livingston: "An essential role for p300/CBP in the cellular response to hypoxia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 23, pp. 12969-73, 1996 (PubMed).

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