MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) antibody

Antigen: MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2)

Synonyms: FCC1, COCA1, HNPCC, LCFS2, HNPCC1, AI788990

Clonality: Monoclonal (25D12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN115118

Additional Information

Alternative name: MSH2

UniProt: P43246

Immunogen: BALB/C mice were injected with recombinant human MSH2 protein.

Cross-Reactivity: Human

Isotype: IgG1

Clone: 25D12

Description: MSH2 protein is involved in the initial recognition of mismatched nucleotides during thepost replication mismatch repair process. Loss of MSH2 function leads to the accumulationof replication errors, which in turn may be responsible for the multiple mutations requiredfor multistage carcinogenesis.

Characteristics: Synonyms: DNA mismatch repair protein Msh2, MutS protein homolog 2

Application Details

Application Notes: Immunohistochemistry on paraffin embedded sections: diluted of 1: 10-1: 25 in an ABC method (60 minutes at room temperature)Recommended Positive Control: TonsilOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: 10 mM citrate buffer, pH 6.0 - containing sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer, DNA/RNA

Restrictions: For Research Use only

Publications

Kolodner, Hall, Lipford et al.: "Structure of the human MSH2 locus and analysis of two Muir-Torre kindreds for msh2 mutations." in: Genomics, Vol. 24, Issue 3, pp. 516-26, 1995 (PubMed).

Fishel, Lescoe, Rao et al.: "The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer." in: Cell, Vol. 75, Issue 5, pp. 1027-38, 1994 (PubMed).

Maliaka, Chudina, Belev et al.: "CpG dinucleotides in the hMSH2 and hMLH1 genes are hotspots for HNPCC mutations." in: Human genetics, Vol. 97, Issue 2, pp. 251-5, 1996 (PubMed).

Palombo, Iaccarino, Nakajima et al.: "hMutSbeta, a heterodimer of hMSH2 and hMSH3, binds to insertion/deletion loops in DNA." in: Current biology : CB, Vol. 6, Issue 9, pp. 1181-4, 1997 (PubMed).