CDC25A antibody

Catalog No. ABIN115128

Product Details anti-CDC25A Antibody

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: This antibody reacts with a 65 kDa protein known as CDC25A.
Cellular Localization: Nuclear membrane.

Purification: Purified

Immunogen: BALB/C mice were injected with purified recombinant CDC25A.

Clone: DCS-120-DCS-121

Isotype: IgG2a

Application Details

Application Notes: Immunflourescence. Immunoprecipitation. Western Blot. Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/50-1/100 in an ABC method (30 minutes at room temperature, requireshigh temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior toimmunostaining)Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: CDC25A (CDC25A Products)

Background: The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1

Gene ID: 993

NCBI Accession: NP_001780

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase