Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) antibody

Details for Product No. ABIN115186
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Antigen
Reactivity
Human
(197), (92), (77), (13), (6), (2), (1)
Host
Mouse
(181), (29), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(11), (10), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(172), (96), (64), (49), (40), (30), (22), (13), (8), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 1 mL
Shipping to United States (Change)
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Catalog No. ABIN115186
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Immunogen BALB/C mice were immunized with recombinant human Caspase 6 prodomain.
Clone 6CSP03
Isotype IgG1
Specificity This antibody stains with a 34 kD protein, known as Caspase 6. Cellular Localization: Cytoplasmic.
Purification Protein G purified ascites
Alternative Name Caspase-6
Background Caspases play a major role in the transduction of the apoptotic signal and execution of apoptosis in mammalian cells. Caspase 3 (CPP32) and Caspase 6 (Mch2) are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis- inducing stimuli and in all cell lines analyzed. Both CPP32 and Mch2 are present in apoptotic cells as multiple active species. Caspase-6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
Alternate names: Apoptotic protease Mch-2, CASP-6, CASP6, MCH-2, MCH2
Gene ID 839
NCBI Accession NP_001217
UniProt P55212
Research Area Apoptosis/Necrosis
Application Notes Immunoprecipitation (Denaturated): use Protein G and 20 l antibody/mg protein lysate. Immunohistochemistry on paraffin embedded sections: 1: 50-1: 100 in an ABC method,incubation period of 30 minutes at room temperature. Formalin fixed paraffin embeddedtissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH6.0 prior to immunostaining. Western Blot (1: 100). Positive Control: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions
Expiry Date 12 months
Background publications Fernandes-Alnemri, Litwack, Alnemri: "Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family." in: Cancer research, Vol. 55, Issue 13, pp. 2737-42, 1995 (PubMed).

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