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Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) antibody

Antigen

Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6)
Synonyms MCH2, Mch2, mCASP-6, MGC93335, xCaspase-6, mch2, caspase-6, CASP6, MGC127633, zgc:112960, casp6
Clonality Monoclonal (6CSP03)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN115187
Quantity
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
Application Details Alternatives Variants back to top

Alternative name Caspase-6
UniProt P55212
Immunogen BALB/C mice were immunized with recombinant human Caspase 6 prodomain.
Cross-Reactivity Human
Isotype IgG1
Clone 6CSP03
Description Caspases play a major role in the transduction of the apoptotic signal and execution ofapoptosis in mammalian cells. Caspase 3 (CPP32) and Caspase 6 (Mch2) are the majoractive caspases in apoptotic cells, and are activated in response to distinctapoptosis-inducing stimuli and in all cell lines analyzed. Both CPP32 and Mch2 are presentin apoptotic cells as multiple active species. Caspase-6 cleaves nuclear mitotic apparatusprotein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
Characteristics Synonyms: CASP-6, CASP6, MCH2, MCH-2, Apoptotic protease Mch-2

Application Details
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Application Notes Immunoprecipitation (Denaturated): use Protein G and 20 l antibody/mg protein lysate. Immunohistochemistry on paraffin embedded sections: 1: 50-1: 100 in an ABC method,incubation period of 30 minutes at room temperature. Formalin fixed paraffin embeddedtissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH6.0 prior to immunostaining. Western Blot (1: 100). Positive Control: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Purification Purified
Buffer PBS containing sodium azide as preservative.
Storage Store the antibody at 2-8°C or in aliquots at -20°C long term. Avoid repeated freezing andthawing. Shelf life: one year from despatch.
Research Area Apoptosis/Necrosis
Restrictions For Research Use only

Alternatives
Application Details Variants back to top

Alternatives for antigen "Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6)", type "Antibodies"
Hosts Rabbit (76), Mouse (13)
Reactivities Human (78), Rat (Rattus) (28), Mouse (Murine) (27), Cow (Bovine) (5), Dog (Canine) (4), Rabbit (2), Cat (Feline) (1), Chicken (1), Gerbil (1), Hamster (1), Monkey (1), Pig (Porcine) (1), Sheep (Ovine) (1)
Applications Western Blotting (WB) (78), ELISA (38), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (IHC) (19), Immunofluorescence (IF) (15), Immunocytochemistry (ICC) (12), Immunoprecipitation (IP) (12), Flow Cytometry (FACS) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2)
Conjugates Biotin (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), FITC (1), PE,Cy5.5 (1)
Epitopes C-Term (9), pSer257 (7), N-Term (6), Internal Region (4), Cleaved-Asp162 (2), Ser257 (2), AA 250-264 (1), C-Term,active (1), Cleavage Site (1), Middle Region (1), Pro-form (1), Prodomain (1), active (1), pSer146 (1)