Epithelial Cell Adhesion Molecule (EPCAM) antibody

Antigen: Epithelial Cell Adhesion Molecule (EPCAM)

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Clonality: Monoclonal (Ber-EP4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN115233

Additional Information

Alternative name: CD326 / EPCAM / TACSTD1

UniProt: P16422

Immunogen: BALB/C mice were immunized with human breast carcinoma cell line, MCF-7.

Cross-Reactivity: Human

Isotype: IgG1

Clone: Ber-EP4

Description: Epithelial antigen is cell surface glycoprotein and is broadly distributed in epithelial cellsand displays a highly conserved expression in carcinomas. Epithelial antigen has beenknow to play an important role as tumor-cell marker in lymph nodes from patients withesophageal carcinoma. Epithelial antigen can be used to distinguish between basal celland basosquamous carcinomas and squamous cell carcinoma of the skin.

Characteristics: Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2,M4S1, MIC18, Tumor-associated calcium signal transducer 1, Major gastrointestinaltumor-associated protein GA733-2, Epithelial glycoprotein 314,Adenocarcinoma-associated antigen, KS 1/4 antigen

Specificity: Recognizes Human Epithelial Antigen. Cellular Localization: Cell membrane. Species: Human. Others not tested.

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Application Details

Application Notes: Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/50-1/150dilution in an ABC method (30 minutes at RT). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Kidney. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Supernatant

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer

Restrictions: For Research Use only

Publications

Momburg, Moldenhauer, Haemmerling et al.: "Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues." in: Cancer research, Vol. 47, Issue 11, pp. 2883-91, 1987 (PubMed).

Beer, Shepherd, Theaker: "Ber EP4 and epithelial membrane antigen aid distinction of basal cell, squamous cell and basosquamous carcinomas of the skin." in: Histopathology, Vol. 37, Issue 3, pp. 218-23, 2000 (PubMed).

Carella, Deleonardi, DErrico et al.: "Immunohistochemical panels for differentiating epithelial malignant mesothelioma from lung adenocarcinoma: a study with logistic regression analysis." in: The American journal of surgical pathology, Vol. 25, Issue 1, pp. 43-50, 2000 (PubMed).

Hosch, Kraus, Scheunemann et al.: "Malignant potential and cytogenetic characteristics of occult disseminated tumor cells in esophageal cancer." in: Cancer research, Vol. 60, Issue 24, pp. 6836-40, 2001 (PubMed).