Melanoma antibody

Antigen: Melanoma

Clonality: Monoclonal (Cocktail)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN115270

Additional Information

Alternative name:

Immunogen: Highly pure (>98%) recombinant hSDF-1-beta (human SDF-1-beta).

Cross-Reactivity: Human

Isotype: Cocktail

Clone: Cocktail

Application Details

Application Notes: ELISA: To detect hSDF-1-beta by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 ug/ml. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthSDF-1-beta. Western Blot: To detect hSDF-1-beta by Western Blot analysis this antibody can be used ata concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hSDF-1-beta is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Supernatant

Buffer: PBS, pH 7.2 without preservatives.Label: Biotin conjugated.Reconstitution: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.

Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibodycan be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Research Area: Cancer

Restrictions: For Research Use only

Publications

Kapur, Bigler, Skelly et al.: "Anti-melanoma monoclonal antibody HMB45 identifies an oncofetal glycoconjugate associated with immature melanosomes." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 40, Issue 2, pp. 207-12, 1992 (PubMed).

Gown, Vogel, Hoak et al.: "Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes." in: The American journal of pathology, Vol. 123, Issue 2, pp. 195-203, 1986 (PubMed).

Chen, Stockert, Tsang et al.: "Immunophenotyping of melanomas for tyrosinase: implications for vaccine development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 18, pp. 8125-9, 1995 (PubMed).

Kawakami, Battles, Kobayashi et al.: "Production of recombinant MART-1 proteins and specific antiMART-1 polyclonal and monoclonal antibodies: use in the characterization of the human melanoma antigen MART-1." in: Journal of immunological methods, Vol. 202, Issue 1, pp. 13-25, 1997 (PubMed).