MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) antibody

Antigen: MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1)

Synonyms: FCC2, COCA2, HNPCC, hMLH1, HNPCC2, MGC5172, MGC66301, zgc:66301, MLH1, MGC140127, LOC100232198

Clonality: Monoclonal (G168-15)

Host: Mouse

Reactivity: Hamster, Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN115274

Additional Information

Alternative name: MLH1

UniProt: P40692

Immunogen: Full length recombinant MLH.

Cross-Reactivity: Hamster, Human, Rat (Rattus)

Isotype: IgG1

Clone: G168-15

Description: The repair of mismatch DNA is essential to maintaining the integrity of genetic informationover time. An alteration of microsatellite repeats is the result of slippage owing to strandmisalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. Theimportance of mismatch repair genes became apparent with the identification of thegenetic basis for hereditary nonpolyposis colon cancer (HNPC). MSH-2 is involved in theinitial cognition of mismatch nucleotides during the replication mismatch repair process. Itis thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimerof MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

Characteristics: Synonyms: DNA mismatch repair protein Mlh1, COCA2

Application Details

Application Notes: Immunohistochemistry on formalin-fixed paraffin embedded sections: Dilute 1/25-1/50 inan ABC method (30 to 60 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil, colon carcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Supernatant

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer, DNA/RNA

Restrictions: For Research Use only

Images

Formalin fixed paraffin embedded human tonsil stained with MLH-1 antibody ABIN115273/ABIN115273-05

Publications

Pearson, Pinkerton, Lewis et al.: "High-dose rapid and standard induction chemotherapy for patients aged over 1 year with stage 4 neuroblastoma: a randomised trial." in: The lancet oncology, Vol. 9, Issue 3, pp. 247-56, 2008 (PubMed).