MLH1 antibody (MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli))

Details for Product anti-MLH1 Antibody No. ABIN115274, Supplier: Log in to see
Antigen
  • CG11482
  • Dmel\\CG11482
  • dmlh-1
  • dmlh1
  • zgc:66301
  • MLH1
  • LOC100232198
  • 1110035C23Rik
  • AI317206
  • AI325952
  • AI561766
  • COCA2
  • FCC2
  • HNPCC
  • HNPCC2
  • hMLH1
  • mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
  • CG11482 gene product from transcript CG11482-RB
  • mutL homolog 1, colon cancer, nonpolyposis type 2
  • mutL homolog 1 (E. coli)
  • MLH1
  • Mlh1
  • mlh1
  • LOC100232198
Alternatives
anti-Human MLH1 antibody for Immunofluorescence (fixed cells)
Reactivity
Hamster, Human, Rat (Rattus)
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Host
Mouse
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77
Clonality (Clone)
Monoclonal ()
Conjugate
This MLH1 antibody is un-conjugated
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Options
Supplier
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Immunogen Full length recombinant MLH.
Clone G168-15
Isotype IgG1
Specificity The G168-15 antibody recognizes MLH1 80-85 kDa.
Cellular Localization: Nuclear
Purification Supernatant
Alternative Name MLH1 (MLH1 Antibody Abstract)
Background The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.Synonyms: COCA2, DNA mismatch repair protein Mlh1
Gene ID 4292
NCBI Accession NP_000240
UniProt P40692
Research Area Cancer, DNA/RNA
Pathways DNA Damage Repair, Production of Molecular Mediator of Immune Response
Application Notes Immunohistochemistry on formalin-fixed paraffin embedded sections: Dilute 1/25-1/50 inan ABC method (30 to 60 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil, colon carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (IHC) image for anti-MLH1 antibody (MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli)) (ABIN115274) Formalin fixed paraffin embedded human tonsil stained with MLH-1 antibody
Product cited in: Pearson, Pinkerton, Lewis, Imeson, Ellershaw, Machin, ,: "High-dose rapid and standard induction chemotherapy for patients aged over 1 year with stage 4 neuroblastoma: a randomised trial." in: The lancet oncology, Vol. 9, Issue 3, pp. 247-56, 2008 (PubMed).