Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) antibody

Antigen: Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1)

Synonyms: flt1b, VEGFR1, FLT, Flt-1, sFlt1, VEGFR-1, AI323757, FLT1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN115390

Additional Information

Alternative name: VEGFR-1 / Flt-1

UniProt: P17948

Immunogen: A synthetic peptide derived from the C-terminus of the Flt-1 precursor of human origin

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosineprotein kinase activity that is important for the control of cell proliferation anddifferentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternativelyspliced form of the gene produces a soluble protein (sFlt1) which binds vascularendothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGFindicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 isspecifically expressed in most vascular endothelial cells and peripheral blood monocytes. Flt-1 is a cell membrane receptor kinase. Flk-1 has a high affinity receptor for vascularendothelial growth factor and is putatively involved in the growth of endothelial cells andangiogenesis.

Characteristics: Synonyms: VEGFR1, FLT1, FLT, FRT, VEGF Receptor 1, Vascular endothelial growth factor receptor 1,Vascular permeability factor receptor, Tyrosine-protein kinase receptor FLT,Tyrosine-protein kinase FRT, Fms-like tyrosine kinase 1

Application Details

Application Notes: Immunohistochemistry on Formalin-Fixed Paraffin Embedded Tissues: Use a dilution of1/50-1/100 in an ABC method (incubation period of 30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0, prior to immunostaining. Recommended Positive Control: Angiosarcoma. Also useful for Western blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Storage: Store the antibody undiluted at 2-8°C. Shelf life: One year from despatch.

Research Area: Cytokines, Receptors

Restrictions: For Research Use only

Images

Formalin fixed paraffin embedded human angiosarcoma stained with Flt-1 antibody ABIN115390 / ABIN115390-05

Publications

Sato, Takino, Okada et al.: "A matrix metalloproteinase expressed on the surface of invasive tumour cells." in: Nature, Vol. 370, Issue 6484, pp. 61-5, 1994 (PubMed).

Sato, Kinoshita, Takino et al.: "Activation of a recombinant membrane type 1-matrix metalloproteinase (MT1-MMP) by furin and its interaction with tissue inhibitor of metalloproteinases (TIMP)-2." in: FEBS letters, Vol. 393, Issue 1, pp. 101-4, 1996 (PubMed).