X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3) antibody

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN115395

Additional Information

Characteristics: Synonyms: DNA repair protein XRCC3, X-ray repair cross-complementing protein 3

Alternative name: XRCC3

Swiss-Prot: O43542

Immunogen: A synthetic peptide derived from the N-terminus of human XRCC3 protein.

Description: XRCC3 is essential for repairing the double strand DNA breaks by homologousrecombination between sister chromatids. It is homologous to Rad1. Five humanhomologues of RAD 51, XRCC2, XRCC3, RAD51B, RAD51C, RAD51D, interact amongthemselves to form one big complex or several smaller complexes.

Specificity: This antibody reacts with a 32 kDa protein, known as XRCC3(X-Ray Cross Complementing 3). Cellular Localization: Nuclear, cytoplasmicSpecies: Human. Others not tested.

Application Details

Application Notes: Western Blot: 1: 100-500. Immunohistochemistry on paraffin embedded sections: diluted of 1: 25-1: 50 in an ABC method (30 minutes at room temperature)Recommended Positive Control: TestisOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: 10 mM citrate buffer, pH 6.0 - containing sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling, Chromatin Binding Proteins, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis of human lung cell extract with XRCC3 antibody. ABIN115394

Publications

Publications: Tambini, George, Rommens et al.: "The XRCC2 DNA repair gene: identification of a positional candidate." in: Genomics, Vol. 41, Issue 1, pp. 84-92, 1997 (PubMed).

Johnson, Liu, Jasin: "Mammalian XRCC2 promotes the repair of DNA double-strand breaks by homologous recombination." in: Nature, Vol. 401, Issue 6751, pp. 397-9, 1999 (PubMed).