Midkine antibody

Catalog No. ABIN115508

Product Details anti-Midkine Antibody

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Midkine antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequence: MKHHHHHHHM KKKDKVKKGG PGSECAEWAW GPCTPSSKDC GVGFREGTCG AQTQRIRCRV PCNWKKEFGA DCKYKFENWG ACDGGTGTKV RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D

Specificity: This antibody reacts to Midkine.

Purification: Immunoaffinity chromatography

Immunogen: Recombinant Human Midkine produced in E.Coli

Application Details

Application Notes: ELISA. Western Blot. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.05 mL of deionized water

Concentration: 1.0 mg/mL

Buffer: 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 months.

Expiry Date: 6 months

References for anti-Midkine antibody (ABIN115508)

Hara, Ikeda, Shimada, Nagamine, Sawa, Oda: "[Surgical experience of 6 multiple primary lung cancers]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 3, pp. 183-7, (2008) (PubMed).

Target Details for Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Alternative Name: Midkine (MDK Products)

Synonyms: ARAP antibody, MK antibody, NEGF2 antibody, Mek antibody, MK-B antibody, PTF-alpha-1 antibody, mdk antibody, midkine antibody, negf2 antibody, RIHB antibody, cb518 antibody, fb79b05 antibody, fj33b02 antibody, fj48a12 antibody, mdk2 antibody, plei2 antibody, wu:fb79b05 antibody, wu:fj33b02 antibody, wu:fj48a12 antibody, MK-A antibody, PTF-alpha-2 antibody, X-PTF-alpha1 antibody, XMK antibody, mdk-a antibody, mdk-b antibody, midkine antibody, midkine L homeolog antibody, midkine (neurite growth-promoting factor 2) antibody, midkine b antibody, midkine S homeolog antibody, MDK antibody, Mdk antibody, mdk.L antibody, mdkb antibody, mdk.S antibody

Background: Midkine (MK, also called neurite growth promoting factor 2, NEGF-2), a product of a retinoic acid responsive gene, is a secreted 13 kDa protein belonging to the family of heparin binding growth/differentiation factors. MK shares 45 % sequence identity with other member of this family called Pleiotrophin (HB-GAM). Midkine is composed of two domains held together by disulfide linkages. The C-terminally located domain contains two heparin binding sites and is usually responsible for midkine activity. Part of the MK activity is enhanced by dimerization of MK. Midkine has been found in vertebrates from human to zebrafish and is most strongly expresed in midgestation. In the adult MK expression is restricted. In addition to normal development, MK is also involved in the pathogenesis of diseases e.g. inflammatory diseases, human carcinomas such as esophageal, stomach, colon, pancreatic, thyroid, lung, urinary, hepatocellular, neuroblastoma, glioblastoma, Wilm´s tumor etc. High MK levels are associated with poor prognosis in some type of cancer. The increased expresion in many carcinomas indicates that MK can be applied to the diagnosis of malignancy. Midkine is expressed during the reparative stage of bone fractures, also supresses infection of certain viruses including HIV in target cells. Anti-apoptotic and cell protecting activity of midkine makes it to be a promissing in therapy.Synonyms: Amphiregulin-associated protein, MDK, MK, MK1, Midgestation and kidney protein, NEGF2, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

Gene ID: 4192

NCBI Accession: NP_001012333

UniProt: P21741

Pathways: RTK Signaling, M Phase, Skeletal Muscle Fiber Development