Pleiotrophin antibody (PTN)

Details for Product anti-PTN Antibody No. ABIN115512, Supplier: Log in to see
Antigen
  • zHBNF
  • zgc:111787
  • harp
  • hbnf
  • X-PTF
  • hbgf8
  • negf1
  • ptn
  • PTN
  • HARP
  • HBGF8
  • HBNF
  • NEGF1
  • PTN-A
  • HB-GAM
  • HBBM
  • HBGF-8
  • OSF-1
  • HBBN
  • OSF
  • Osf-1
  • Osf1
  • Hbnf
  • pleiotrophin
  • Pleiotrophin
  • pleiotrophic factor beta
  • pleiotrophin-like
  • ptn
  • PTF-BETA
  • PTN
  • Ptn
  • LOC100351242
Alternatives
anti-Human Pleiotrophin antibody for Enzyme Immunoassay
Reactivity
Human
114
39
33
9
4
4
4
4
3
2
1
Host
Rabbit
98
16
11
4
Clonality
Polyclonal
Conjugate
This Pleiotrophin antibody is un-conjugated
13
8
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
108
64
46
30
16
13
6
4
4
2
1
Options
Supplier
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Immunogen Recombinant human OSF-1 (E.coli)
Sequence MKHHHHHHHM LVPRGSGKKE KPEKKVKKSD CGEWQWSVCV PTSGDCGLGT REGTRTGAEC KQTMKTQRCK IPCNWKKQFG AECKYQFQAW GECDLNTALK TRTGSLKRAL HNAECQKTVT ISKPCGKLTK PKPQAESKKK KKEGKKQEKM LD
Specificity This antibody reacts to Pleiotrophin.
Purification Immunoaffinity chromatography
Alternative Name Pleiotrophin / PTN (PTN Antibody Abstract)
Background Pleiotrophin (Osteoblast-Specific Factor-1, OSF-1) contains 136 amino acid residues. The sequence is very rich in cationic amino acids (24 % of the residues), lysine cluster sequences are found in the N-terminal and Cterminal ends of the structure. The OSF-1 gene was shown by Northern blotting analysis to be expressed in mouse calvarial osteoblastenriched cells and in mouse brain tissues, but not in thymus, spleen, kidney, liver, lung, testis or heart. Pliotrophin has the ability to promote adhesion, migration, expansion, and differentiation of human osteoprogenitor cells. In addition to certain types of cancer, the embryonic growth and differentiation factor pleiotrophin is found also in adults in inflammatory diseases. In osteoarthritis, pleiotrophin is especially expressed in early stages, and its concentrations in the synovial fluid could serve as a marker for the progress of the disease. Pleitrophin might be involved in cartilage repair in osteoarthritis, in particular, in earlier stages. Total 152 AA. MW: 17.3 kDa. N-Terminal His-tag and thrombin cleavage site 16 AA (highlighted).Synonyms: HB-GAM, HBBM, HBGF-8, HBNF-1, HBNF1, Heparin-binding brain mitogen, Heparin-binding growth factor 8, Heparin-binding growth-associated molecule, Heparin-binding neurite outgrowth-promoting factor 1, NEGF1, OSF-1, OSF1, Osteoblast-specific factor 1
Gene ID 5764
NCBI Accession NP_002816
UniProt P21246
Pathways RTK Signaling
Application Notes ELISA. Western blot. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.05 mL of deionized water.
Concentration 1.0 mg/mL
Buffer 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Product cited in: Asari, Kanemitsu, Kurihara: "Oral administration of high molecular weight hyaluronan (900 kDa) controls immune system via Toll-like receptor 4 in the intestinal epithelium." in: The Journal of biological chemistry, Vol. 285, Issue 32, pp. 24751-8, 2010 (PubMed).

Background publications Ulbricht, Brockmann, Aigner, Eckerich, Müller, Fillbrandt, Westphal, Lamszus: "Expression and function of the receptor protein tyrosine phosphatase zeta and its ligand pleiotrophin in human astrocytomas." in: Journal of neuropathology and experimental neurology, Vol. 62, Issue 12, pp. 1265-75, 2003 (PubMed).

Hida, Jung, Wu, Kim, Kodama, Masuda, Nishino: "Pleiotrophin exhibits a trophic effect on survival of dopaminergic neurons in vitro." in: The European journal of neuroscience, Vol. 17, Issue 10, pp. 2127-34, 2003 (PubMed).

Muramatsu: "Midkine and pleiotrophin: two related proteins involved in development, survival, inflammation and tumorigenesis." in: Journal of biochemistry, Vol. 132, Issue 3, pp. 359-71, 2002 (PubMed).

Deuel, Zhang, Yeh, Silos-Santiago, Wang: "Pleiotrophin: a cytokine with diverse functions and a novel signaling pathway." in: Archives of biochemistry and biophysics, Vol. 397, Issue 2, pp. 162-71, 2002 (PubMed).

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