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Podoplanin antibody (PDPN)

Details for Product anti-PDPN Antibody No. ABIN115686, Supplier: Log in to see
Antigen
  • AGGRUS
  • E11
  • GP36
  • Gp38
  • GP40
  • HT1A-1
  • OTS-8
  • OTS8
  • PA2.26
  • PDPN
  • RANDAM-2
  • RTI40
  • T1-alpha
  • T1A
  • T1a
  • T1A-2
  • T1alpha
Reactivity
Human
240
139
29
3
Host
Rabbit
104
98
92
40
22
13
4
1
Clonality
Polyclonal
Conjugate
This Podoplanin antibody is un-conjugated
22
16
15
13
11
11
10
9
9
9
9
9
9
8
8
7
6
6
5
4
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
278
161
124
85
84
61
53
46
39
11
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Immunogen Recombinant ectodomain of human gp36 (Podoplanin)
Specificity This antibody detects Podoplanin. The antibody is not reactive with mouse podoplanin.
Purification Protein A affinity chromatography
Alternative Name Podoplanin (PDPN Antibody Abstract)
Background Podoplanin is a highly O-glycosylated integral membrane protein that is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, Podoplanin colocalized with VEGFR-3/FLT4, another marker for lymphatic endothelial cells.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha
Gene ID 10630
NCBI Accession NP_001006625
UniProt Q86YL7
Application Notes Immunoprecipitation: 2-4 μg/sample. Immunofluorescence: 1-2 μg. Western blotting: 1-2 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in sterile water to a concentration of > 0.5 mg/mL.
Buffer PBS, pH 7.4, without preservative or stabilizer
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment The lyophilized IgG is stable at 2-8 °C for one month and for one year from despatch when kept at -20 °C. When reconstituted in sterile water to a concentration of > 0.5 mg/ml the antibody is stable for six weeks at 2-8 °C in the presence of a preservative. Reconstituted antibody can also be aliquotted and stored at -20 °C to -70 °C for six months without detectable loss of activity.
Expiry Date 12 months
Product cited in: Conrad, Niess, Huss, Huber, von Luettichau, Nelson, Ott, Jauch, Bruns: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." in: Circulation, Vol. 119, Issue 2, pp. 281-9, 2009 (PubMed).

Background publications Schacht, Ramirez, Hong, Hirakawa, Feng, Harvey, Williams, Dvorak, Dvorak, Oliver, Detmar: "T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema." in: The EMBO journal, Vol. 22, Issue 14, pp. 3546-56, 2003 (PubMed).

Zimmer, Oeffner, Von Messling, Tschernig, Gröness, Klenk, Herrler: "Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium." in: The Biochemical journal, Vol. 341 ( Pt 2), pp. 277-84, 1999 (PubMed).

Breiteneder-Geleff, Matsui, Soleiman, Meraner, Poczewski, Kalt, Schaffner, Kerjaschki: "Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis." in: The American journal of pathology, Vol. 151, Issue 4, pp. 1141-52, 1997 (PubMed).