This antibody will detect Prox-1 in the nucleus of lymphatic endothelial cells by Immunostaining. This antibody cross reacts with Equine Prox-1 in Immunohistochemistry using Paraffin-Embedded Sections (Figure 2).
Purification
Antigen-Affinity Chromatography.
Immunogen
Recombinant Human C-terminal part of the Homeobox Transcription Factor Prox-1 (homeo domain and prospero domain).
Immunofluorescence: Use 1/750-1/1000 dilution. Western Bloting: Use 1-5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with distilled sterile water to a concentration of 0.1-1.0 mg/mL
Buffer
5 mM PBS, pH 7.2 without preservatives
Preservative
Without preservative
Handling Advice
Dilute only prior to immediate use
Storage
4 °C
Storage Comment
Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lymphatic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells.Synonyms: Homeobox prospero-like protein PROX1, PROX1, Prospero homeobox protein 1