Histidine Decarboxylase (HDC) antibody

Antigen: Histidine Decarboxylase (HDC)

Synonyms: MGC163399, Hdc-a, Hdc-c, Hdc-e, Hdc-s, AW108189, 4732480P20, MGC156577, JK910, anon-WO0140519.259, hdc, DmelCG3454, CG3454, HDC, LOC100313635

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN115730

Additional Information

Characteristics: Synonyms: HDC

Alternative name: Histidine decarboxylase

Swiss-Prot: P19113

Immunogen: Recombinant Histidine Decarboxylase produced in E. coli

Format: Serum

Description: Histidine decarboxylase(HDC) is the enzyme that calalyses the conversion of histidine intohistamine. It can be found in the histamine secreting ECL cells of some species, as well asin the mast cells. The biogenic amine histamine is an important modulator of numerousphysiological processes, including neurotransmission, gastric acid secretion and smoothmuscle tone.

Specificity: This antibody reacts with HDC. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining. Species Reactivity: Tested: Human, Rat, Mouse, Canine (Dog) and Guinea-Pig.

Application Details

Application Notes: Immunofluorescence Microscopy: 1/1000-1/1500 with overnight incubation at 2-8°C. Immunohistochemistry on Frozen and Paraffin Embedded Tissues. Positive Control: Stefanini-fixed frozen sections of Rat fundus. Western blot: The antiserum detects the 54 kD and 73 kD forms in addition to a 63 kD form(Rat stomach - see Dartsch et al. , 1998). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Storage: Store the antibody at 2-8°C (Lyophilized) or reconstituted in small aliquots at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Metabolism

Restrictions: For Research Use only

Publications

Publications: Zhao, Jacobsson, Chen et al.: "Exocytotic proteins in enterochromaffin-like (ECL) cells of the rat stomach." in: Cell and tissue research, Vol. 290, Issue 3, pp. 539-51, 1998 (PubMed).

Dartsch, Persson: "Recombinant expression of rat histidine decarboxylase: generation of antibodies useful for western blot analysis." in: The international journal of biochemistry & cell biology, Vol. 30, Issue 7, pp. 773-82, 1998 (PubMed).

Dartsch, Sundler, Persson: "Antisera against rat recombinant histidine decarboxylase: immunocytochemical studies in different species." in: The Histochemical journal, Vol. 31, Issue 8, pp. 507-14, 1999 (PubMed).

Rozenberg, Sluka, Rohrer et al.: "Histamine H1 Receptor Promotes Atherosclerotic Lesion Formation by Increasing Vascular Permeability for Low-Density Lipoproteins." in: Arteriosclerosis, thrombosis, and vascular biology, 2010 (PubMed).